CarbonylsbyCARBMethod1004onanAllure®AKcolumn.
HPLC COLUMNS
Allure® Silica and AK Columns
21
Phone: 800-356-1688 or 814-353-1300
Allure®SilicaColumns (USPL3)
ChromatographicProperties:
Highly retentive phase for normal phase separations.Veryhigh surface area,TypeB silicapacking.
particle size: 3µmor 5µm, spherical
pore size: 60Å
endcap: no
pH range: 2.5 to7.5
temperature limit: 80°C
PhysicalCharacteristics:
1.0mm ID
2.1mm ID
3.2mm ID
4.6mm ID
Length
cat.#
price
cat.#
price
cat.#
price
cat.#
price
3µmColumns
30mm
9160331
$368
9160332
$347
9160333
$347
9160335
$347
50mm
9160351
$368
9160352
$347
9160353
$347
9160355
$347
100mm
9160311
$395
9160312
$372
9160313
$372
9160315
$372
5µmColumns
30mm
9160531
$342
9160532
$319
9160533
$319
9160535
$319
50mm
9160551
$342
9160552
$319
9160553
$319
9160555
$319
100mm
9160511
$368
9160512
$347
9160513
$347
9160515
$347
150mm
9160561
$395
9160562
$372
9160563
$372
9160565
$372
200mm
9160521
$422
9160522
$398
9160523
$398
9160525
$398
250mm
9160571
$447
9160572
$427
9160573
$427
9160575
$427
Allure®AKColumns
ChromatographicProperties:
This highly retentive, highly selective phase, unique toRestek,was developed specifically for the analysis of aldehy-
des andketones asDNPHderivatives.Allure®AK is a reversedphaseHPLCmaterial that has theunique ability to
separate all thirteen carbonyl compounds specified inCaliforniaAirResources Board (CARB)Method#1004,
using a simple acetonitrile/water gradient, in less than15minutes.Other columns require long analysis times or
theuse of tetrahydrofuran.
particle size: 5µm
pore size: 60Å
endcap: yes
pH range: 2.5 - 7.5
temperature limit: 80°C
PhysicalCharacteristics:
3.2mm ID
4.6mm ID
Length
cat.#
price
cat.#
price
5µmColumnswith Trident
™
Integral Inlet Fittings
200mm
9159523-700
$541
9159525-700
$541
new
!
Application
Page#
Carbonyls . . . . . . . . . . . .21, 69
FrankDorman
Director ofTechnical
Development
11+yearsof service!
LC_EV0393
Ret. Time
Peak
(min.)
DNPH derivative of:
1. formaldehyde
4.74
2. acetaldehyde
5.78
3. acrolein
6.86
4. acetone
7.09
5. propionaldehyde
7.31
6. crotonaldehyde
8.19
7. methacrolein
8.55
8. butyraldehyde
8.79
9. methylethylketone
9.06
10. benzaldehyde
10.03
11. valeraldehyde
10.39
12.
m
-tolualdehyde
11.08
13. hexaldehyde
11.36
Sample:
Sample:
dinitrophenylhydrazine (DNPH)
derivatives of aldehydes/ketones
Inj.:
10µL
Conc.:
3µg/mL each analyte, as
aldehyde/ketone
Sample diluent: acetonitrile
Column:
Allure
®
AK
Cat.#:
9159525-700
Dimensions:
200 x 4.6mm
Particle size:
5µm
Pore size:
60Å
Conditions:
Mobile phase:
A) water : B):acetonitrile
Time (min.)
%B
0
60
8
70
10
100
Flow:
1.5mL/min.
Temp.:
30°C
Det.:
UV@ 360nm
