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11
Figure 10
0.32mm ID FAMEWAX
™
Columns AllowMoreConcentratedSamples to be Analyzed in Less Than 20Minutes
1. C14:0
2. C16:0
3. C161n7
4. C16:2n4
5. C16:3n4
6. C16:4n1
7. C18:0
8. C18:1n9
9. C18:1n7
10. C18:1n5
30m, 0.32mm ID, 0.25µmFAMEWAX
™
(cat.#12498). 0.5µl each split injection ofmenhaden oil PUFA&Omegawax
®
testmix.
On-column concentration:
200-350ng.
Oven temp.:
190°C (hold4min.) to 225°C@4°C/min. (hold15min.);
Inj./det. temp.:
225°C/250°C;
Carrier gas:
helium;
Linear velocity:
35cm/sec. @190°C;
FID sensitivity:
8 x 10
-11
AFS;
Split ratio:
50:1.
min.
4
6
8
10
12
14
16
18
11. C18:2n6
12. C18:2n4
13. C18:3n6
14. C18:3n4
15. C18:3n3
16. C18:4n3
17. C18:4n1
18. C20:0
19. C20:1n9
20. C20:1n7
21. C20:2n6
22. C20:3n6
23. C20:4n6
24. C20:3n3
25. C20:4n3
26. C20:5n3
27. C22:0
28. C22:1n9
29. C22:2n6
30. C21:5n3
31. C23:0
32. C22:4n6
33. C22:5n6
34. C22:5n3
35. C24:0
36. C22:6n3
37. C24:1n9
The 0.32mm ID FAMEWAX
™
column alsomeets the resolution criteria for PUFA analysis in less time. Plus, the larger
inner diameter columns provide 4 to 5 timesmore sample capacity (400–500ng vs. 50–100ng) tominimize overloading
ofmore concentrated sampleswithout losing column efficiency.
Figure 10
shows an analysis ofmenhaden oil FAMEs
completed in less than 19minutes on a 30m, 0.32mm ID, 0.25µm FAMEWAX
™
column.
30
32
31
36
35
37
15 16 17 18 19
33
29
34
14
33
34
35
36
37
29
30
31
32
9
10
11
12
13
14
15
16
17
18
19
20
21 22
23
24
25
26
27
28
1
2 3
4
5
6
7
8
