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4
Stabilwax
®
Columns for ComplexMixtures
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ThisPEG columnalsoprovides anaffinity towardand separation ofmanypolar compounds found in complex samples
such as spearmint oil and industrial solventmixtures. (
Figures 2
and
3
)
Figure 2
Flavor Components of Native Spearmint Oil are Baseline Resolved on a Stabilwax
®
Column
1 2
3
4
5 8
9
12
10
11
13
14
15
16
17
18
19
20
21
22
23
24
26 27
28
29
30
25
c-gram #97
60m, 0.25mm ID, 0.25µmStabilwax
®
(cat.#10626)
0.2µl split injection of aneat spearmint oil.
Oven temp.:
75°C (hold4min.) to 200°C@4°C/min. (hold10min.)
Inj. & det. temp.:
250°C;
Carrier gas:
hydrogen;
Linear velocity:
40cm/sec. set @160°C;
FID sensitivity:
4 x 10
-11
AFS;
Split ratio:
100:1
6
8 10 12 14 16 18 20 22 24 26 28 30 32
min. 4
6 7
Figure 3
Industrial Solvents Separated on a Stabilwax
®
Column inUnder 10minutes
15
14
c-gram #2002
1.pentane
2.hexane
3.methyl formate
4.acetone
5. tetrahydrofuran
6.1,1,1-trichloroethane
7.ethyl acetate
8.methanol
9.methylethyl ketone
10.methylene chloride
30m, 0.53mm ID, 1.0µmStabilwax
®
(cat.#10655)
Wet needle injection of neat solvents.
Oven temp.:
50°C to 220°C@10°C/min.;
Inj. & det. temp.:
250°C;
Carrier gas:
helium;
Linear velocity:
20cm/sec.;
FID sensitivity:
5.12 x 10
-10
AFS
3
4
5
8,9
6,7
2
1
10,11
16
13 17
18
19
20
12
min. 2
4
6
8
10
11. chloroform
12.benzene
13.n-propyl alcohol
14. sec-butyl alcohol
15. toluene
16.n-butyl acetate
17. isobutyl alcohol
18.n-butyl alcohol
19.
o
-xylene
20. ethoxyethane
1.
α
-pinene
2.
β
-pinene
3. sabinene
4. myrcene
5.
α
-terpinene
6. L-limonene
7. 1,8-cineole
8.
cis
-ocimene
9.
γ
-terpinene
10.
p
-cymene
11. terpinolene
12. 3-octyl acetate
13. 3-octanol
14. L-menthone
15.
trans
-sabinenehydrate
16.
β
-bourbonene
17. linalool
18. terpinene-4-ol
19.
β
-caryophyllene
20. dihydrocarvone
21.
trans
-dihydrocarvyl
acetate
22.
trans
-
β
-farnesene
23.
α
-terpineol
24. germacrene-D
25. carvone
26.
cis
-carvyl acetate
27.
trans
-carveol
28.
cis
-carveol
29.
cis
-jasmone
30. viridiflorol
