CLINICAL/FORENSICS
47
HPLC Columns
Pinnacle™ IIPhenyl (USPL11)
PhysicalCharacteristics:
particle size: 3µmor 5µm, spherical
endcap: fully endcapped
pore size: 110Å
pH range: 2.5 to10
carbon load: 6%
temperature limit: 80°C
ChromatographicProperties:
ThePinnacle™ II Phenyl phase offers unique selectivity versus traditional alkyl chainphases, especially for
aromatic compounds.
ChoosingaStationaryPhase
Identifying the appropriate stationaryphase for your separation is themost critical stepof column selec-
tion. The choice of stationary phase should be based on sample solubility and on chemical differences
among the sample compounds that canbe exploited to separate the analytes of interest (Figure 1).
Sample hydrophobicity also is amajor determinant of the separationmode (reversed phase or normal
phase). In reversedphase separations, themobile phase ismorepolar than the stationaryphase,which is
traditionally a straight alkyl chain,most oftenoctadecylsilyl,C18 (ODS).ThemajorityofHPLC analyses
are performed in reversedphasemode becausemost analytes of interest canbe dissolved inmixtures of
water and/or apolar organic solvent such asmethanol or acetonitrile.
Innormal phase separations, themobilephase is less polar than the stationaryphase (e.g.,whenbare sil-
ica is the stationary phase and hexane with amodifier is themobile phase). The normal phasemode is
usedprimarilywhen the reversedphasemode cannot beused, because reversedphase separations gener-
ally aremore robust, reproducible, and versatile. Note that a stationary phase incorporating both polar
and nonpolar functionality can be used in either reversed phase or normal phasemode. Cyano phases
(e.g.,Ultra, Pinnacle™DB, or Pinnacle™ II cyano columns) commonly areused in either reversedphase
or normal phasemode.Ultra IBD andAllure™Basix columns also canbeused for either reversedphase
ornormal phase separations.Allure™PFPPropyl andUltraPFP columns alsodisplaydual functionality.
Figure1
Choiceof stationary
phase isdependent
uponpolarityof the
sample.
Non-polar analytes are
more attracted to the
less polar stationary
phase. Polar analytes
aremore strongly
retainedby themore
polar stationaryphase.
Choose thebest stationaryphase for your applicationbasedonanalyte functionality.
Neutral
Base
Acid
Mixed
Acids, Bases, &Neutrals
AnalyteFunctionalGroup
Ultra IBD
Ultra Aqueous C18
Allure
™
Aqueous C18
Allure
™
Organic Acids
Allure
™
Basix
Allure
™
PFP Propyl
Ultra IBD
Ultra PFP
Pinnacle
™
DB Cyano
Ultra Cyano
Ultra Amino
Pinnacle
™
II Amino
Pinnacle
™
DB C18
Ultra C18
Pinnacle
™
II C18
Allure
™
C18
Ultra C8
Pinnacle
™
II C8
Pinnacle
™
II Phenyl
Viva C18
Allure
™
Biphenyl
Ultra IBD
Ultra Aqueous C18
Allure
™
Aqueous C18
Allure
™
Organic Acids
Ultra IBD
Allure
™
Basix
Hydrophobic
Hydrophilic
Toordera2.1mm, 3.2mm, or4.6mm IDcolumnwithaTrident
™
Integral InletFitting,
add“-700”to thecatalognumber for thecolumn.
Example: 100mm x 4.6mm IDUltraC18 columnwithTrident
™
Integral Inlet Fitting: 9174315-700
Nominal additional charge
$15.00
Forguardcartridges for thesecolumns, seepage52.
1.0mm ID
2.1mm ID
3.2mm ID
4.6mm ID
Length
cat.#
price
cat.#
price
cat.#
price
cat.#
price
3µmColumns
30mm 9215331
$260
9215332
$240
9215333
$240
9215335
$240
50mm 9215351
$276
9215352
$256
9215353
$256
9215355
$256
100mm 9215311
$291
9215312
$271
9215313
$271
9215315
$271
5µmColumns
30mm 9215531
$250
9215532
$230
9215533
$230
9215535
$230
50mm 9215551
$266
9215552
$246
9215553
$246
9215555
$246
100mm 9215511
$281
9215512
$261
9215513
$261
9215515
$261
150mm 9215561
$296
9215562
$276
9215563
$276
9215565
$276
