○
○
○
○
○
○
○
○
○
○
○
○
○
○
○
○
4
Figure 2
Restek’s chiral columns demonstrate exceptional lifetime and stability for
more than 250 injections without loss of resolution.
min. 10
20
30
min. 10
20
30
1,2
3
4
8
9
5
6
7
10 11
12
13
14
15
1,2
3
4
8
5
6
7
10 11
12
13
14
15
9
Chiral chromatography is the
separationof enantiomeric
compounds. Common liquid
stationaryphasesdonot
possess adequate selectivity
for enantiomeric separation.
Additionof derivatized
cyclodextrinmacromolecules
tocommon stationaryphases
creates capillary columnswith
the ability to separate
manyenantiomers.
1. (-)
α
-pinene
2. (+)
α
-pinene
3. decane
4. (-) 2,3-butanediol
5. (+) 2,3-butanediol
30m, 0.32mm ID, 0.25µmRt-
β
DEXsa (cat.# 13108)
Oven temp.:
40°C (hold 1min.) to 230°C@ 2°C/min.;
Carrier gas:
hydrogen
;
80cm/sec. set @ 40°C;
Detector:
FID set @ 220°C
WHAT ISCHIRAL
CHROMATOGRAPHY?
○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○
Chiral chromatography is thesepara-
tion of enantiomeric compounds.
Common liquid stationary phases
used in gas chromatography resolve
components from one another, but
they do not possess adequate selec-
tivity for enantiomeric separation.
Addition of derivatized cyclodextrin
macromolecules to common station-
aryphases creates capillary columns
with the ability to separate enanti-
omers as well.
Thepermethylatedderivativeof beta-
cyclodextrin in cyanopropyl-dim-
ethylpolysiloxane liquid stationary
phase iscommonlyused forsuchste-
reochemicalseparations,but itexhib-
its limited applications. Beta-
cyclodextrins derivatized with alkyl
substituents can enhance the enan-
tiomeric resolution of various com-
pound classes. Restek’s five capillary
columns incorporate various combi-
nations of alkylated beta-cyclodex-
trins into a cyanopropyl-dimethyl
polysiloxane liquid stationary phase
to achieve significant separation.
These columns also exhibit stability
and extended lifetime. From the first
injection to the 250th injection on a
chiral column, enantiomeric separa-
tion ismaintainedwithalmostno loss
in resolution (
Figures 2A and B
).
A: 1st injection
B: 250th injection
6. undecane
7. nonanal
8. 1-octanol
9. 2,6-dimethylphenol
10. (+) phenylethanol
11. (-) phenylethanol
12. methyl decanoate
13. dicyclohexylamine
14. methyl undecanoate
15. methyl dodecanoate
