~~~;~~I!I
Fast
GC-MS
analysis of the kunzea essential oilwas alsoperformed
~
.usingtnls configuration anda typical cnrornatoqram isshown inFigure
1
12 .
Note themaximum intensityof the small peakmarked
#
inFigure
2,
I
wnlcn hasa low
retatve
peak intensityof
1.09%,
ismorethan
19x
the
averagebaseline responsebetween 2and5min. To compare the
I
general selectivityof the
MEGA-Wax
HTcolumn, a fast
GC-MS
.I.
II
J
II
II.
t
chromatogram of kunzea essential oil usinga
20
m
x 100
urn i.d.
I·
,.
r_:"
•
Rtx-WAX
column isshown inFigure 3. Temperature effectsaswell as
Figure 3
differences in the stationaryphasechemistry each influencethe
absolute retentionorder soanattemptto overlaythese chromatograms would bepointless. The
MEGA-WAX
HTcolumn isalso75% shorter than the
Rtx-\fl/AX
column, sothe resolution usingthe
latter column isunderstandablybetter. Nonetheless, elutionof themonoterpene and
sesquiterpene hydrocarbons, followed by themonoterpene and sesquiterpene alcohols is in
general congruence between the twocolumns.
AcKnowleagemems
This workwas supported by the Australian research Council's Discovery funding scheme (project
number DP0771893) . The authors wish to thank Dr Stefano Galli (Mega) for providing
MEGA-WAX HT columns, Dr Christian Narcowicz (University of Tasmania) for supplying the
kunzea essential oil sample. The generous support from Restek Corporation is gratefully
acknowledged.
Philip Marriott
is professor of Separation Science at RMIT University, and deputy director of
ACROSS. His interests are in high resolution separations, particularly GC, GCxGC and capillary
electrophoresis, with mass spectrometry amongst other detection techniques .
Paul Morrison
is research technical associate in the Australian Centre for Research on
Separation Science (ACROSS) group at RMIT. He implements high resolution separation
strategies and develops strategic instrumental capabilities within ACROSS.
Samuel Poynter
is a PhD student in ACROSS at University of Tasmania (UTAS).
Robert Shellie
is senior lecturer at UTAS. He leads a research group in ACROSS (UTAS) that
focuses on development and application of hyphenated techniques in chromatography to solve
complex separation problems.
References
1 RA Sh II '
C
HRO~---I.J'~I~+61(0)397622034
Australian Distributors
. . .
e Ie,
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&
Manu'acurers
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2. L. Mondello et
ar.,
r ievour rragr
J.,
::'::::U~L),I
ob-14U
~LUU:J).
3. R. Shellie, P.Marriott and C. Cornwell,
J.
Sep. sot,
24(10-1 '1),823-830 (2001).
4. R. Shellie and P.J.Marriott, Ana!. Chern., 74(20), 5426-5430 (2002) .
5. M. Wang et al.,
J.
Chromatogr A, 1112(1-2), 361-368 (2006).
6. M. Junge et aI., Anal. Chern., 79(12), 4448-4454 (2007) .
7. J.B. Ptlillips and J. Beens,
J.
Chromatogr A, 856(1-2),331-347 (1999).
8. P.Marriott et al., Flavour Fragr
J.,
15(4), 225-239 (2001).
9. J. Thomas et at., Vet. Rec.,
164,
619-623
(2nnq~
__
=
H
RO~---I.J'~I~
+61(0)3 97622034 Australian Distributors
ImpOl1ers
&
Manufacurers
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Website NEW :
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