Recently, we received a 5mx 100umi.d.MEGA-WAXHTnlqh-ternperaturewaxcolumnWittl a
stationary phase filmthickness of 0.10
urn
(Mega, Legnano, Italy) and have been using this
column for our GCxGCwork involvingessential oilsanalysis. Thewaxseconddimension column
used in the current investigationhasmorethan 9000
[\JIm
tested in isothermalmodeand an
upper column oven limitof 300 °C.Results fromthe GCxGCanalysisof kunzea essential oil
obtained by steamdistillationof aerial parts of
Kunzea ambigua
are reported
here
Results
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Kunzea ambigua
is a shrub native to the Easternparts ofAustralia.As
an effective killer of bacteria, kunzeaessential oil has numerous
traditional folkmedicineuses and researchers have recently
developed a kunzea oil formulation for veterinary use? Kunzea oil is
rich in sesquiterpene alcoholsand as a consequence one-dimensional
GC lacks the resolvingpower to provide adequate separation of the
complex oil. Inthe present investigationaGCxGCcolumn set
L.---------"=------'comprising a 30mx 250 urn i.d.RXi-5Sil MS column witha stationary
Figure 1
phase filmthickness of 0.25
urn
(Restek, Bellefonte, Pennsylvania,
USA) was installedas the first dimensionseparation column and the seconddimensioncolumn
wasaimx 100umi.d.MEGA-WAXHTcolumn v....ith a stationary phase filmthlckness of 0.10
~JtTI .
The primarygoal of this investigationwasto determinethe SUitability of theMEGA-WAXHT
column as a second-dimensioncolumn for essential oil analysis, inparticular for providlnq
adequate separation of the sesquiterpene alcohols in kunzea oil. To date,wehavenot attempted
comprehensive peak identification of the essential oil componentsusingGCxGC-MS but we
anticipate that these resultswill be reported in time.
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Prior to removing a 1.0mlength fromthe tvlEGA-WAXHTcolumn, a
seriesof fast GC-MS analyseswereperformed using the 5mcolumn
as recelved.All analyseswereperrormedusing aSllimaejzu GC-MS–
QP2010-Plus (Shimadzu Oceania,Melbourne,Australia). Afast
temperature proqramrned qraolent from40 °Cto 300 °C in 6.5min(40
°C/min)wasemployedfor these analyses and the ovenwasheld at the
maximum temperature for 1minat the eno of the temperature
programme. The
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+61(0)397622034 Australian Distributors
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Importers & Manufacurers
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Web slle NEW :
E·mall : In'o@Chromlech.nel.au Tel : 03 9762 2034 ••• In AUSTRALIA
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performed to assess the level of stationary phase bleed.Evenusinga reasonably aggressive
rapid temperature programme rate up to 300 °Cthese results are highly satisfactory. The
average baseline response (n
=
10) at the maximum oven temperature (6.5-7.5min)was less
than 7.5x tne average baseline response recordeo between2 and 5min.
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+61(0)3 97622034 Australian Distributors
Importers
&
Manufacurers
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ECI-I" " ' 47g .J!"Pty Lld
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