0.0
2.0
4.0
6.0
8.0
10.0 12.0 14.0 16.0 18.0 20.0
1
2
3
4 5
6
7
mfr. H
mfr. D
mfr. C
mfr. F
Viva
™
*
Figure2
Viva™C18: best overall retentionandpeak symmetry.
0.0 1.3 2.5 3.8 5.0 6.3 7.5 8.8 10.0 11.3 12.5 13.8 15.0
1
2 3
4
5
mfr. H
mfr. D
mfr. C
mfr. F
Viva
™
Figure3
Viva™C18: excellent resolutionof peptides.
150 x 2.1mm C18 phase columns, 5µm particles; reversed phase test mix
Mobile Phase: water:methanol, 25:75 v/v; FlowRate: 0.2mL/min.; Detection: UV, 254nm; Sample: 10µL HPLC
Performance (NIST 870)Test Mix (cat.# 31699)
150 x 2.1mm C18 phase columns, 5µm particles; peptide test mix.
Mobile Phase: A - 0.08% trifluoroacetic acid inwater (pH 2.0), B - 0.08%TFA in acetonitrile, 10%B to 40%B in
20min.; FlowRate: 0.2mL/min.; Detection: UV, 214nm; Injection: 20µL eight peptide test mix
*High levels of tracemetals (~100ppm) in the silica enhance this separation.
Figure4
Viva™C18: superior performancewithproteins.
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 22.5 25.0 27.5 30.0
1
2
3
4
mfr. H
mfr. D
mfr. C
mfr. F
Viva
™
150 x 2.1mm C18 phase columns, 5µm particles; four-protein test mix.
Mobile Phase: A - 0.1%TFA inwater, pH 2.0, B - 0.1%TFA in acetonitrile, 20%B to 70%B in 30min.;
FlowRate: 0.2mL/min.; Detection: UV, 214nm; Injection: Four Protein StandardTest Mix
1. val-tyr
2. val-tyr-val
3. meth enkephalin
4. val-4-
angiotensin III
5. angiotensin
II, III
6. leucine
enkephalin
7. angiotensin I
1. ribonuclease A
2. cytochrome c
3. holo-transferrin
4. apomyoglobin
1. uracil
2. toluene
3. ethylbenzene
4. quinizarin
5. amitriptyline
