Chromatography Columns 2013+ - page 218

Figure1
As columnactivity increases, signal decreasesand retention time shifts.
LowerDetectionLimitswithGround-BreakingColumnTechnology
Rxi® columns deliver more accurate, reliable trace-level results than any other fused silica
columnon themarket.Toensure thehighest levelof performance,allRxi®capillarycolumns
are manufactured and individually tested to meet stringent requirements for exceptional
inertness, lowbleed, andunsurpassed column-to-column reproducibility.
Highest Inertness
Inertness is one of themost difficult attributes to achieve in an analytical column, but it is
oneof themost critical as it improvespeak shape, response,and retention time stability.Rxi®
technologyproduces themost inert columns available, providing:
• Increased signal-to-noise ratios to improve low-level detection.
• Reproducible retention times for positive identifications.
• Improved response for polar, acidic, andbasic compounds.
Highest Inertness
LowestBleed
Exceptional Reproducibility
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2
IncreasedSignal andReproducibleRetentionTimes
When capillaries are not sufficiently deactivated, peaks become asymmetric, resulting in
reduced signal andunpredictable retention times.As column activity increases, peak tailing
becomesmorepronounced, reducingpeakheight andcausing retention time todrift (Figure
1). In practice, thismeans that sensitivity is lost and trace-level analytes cannot be reliably
determined. In addition, even compounds at higher concentrations may be misidentified,
due to retention time shifting.
Amore significant problem for sample analysis is that retention time can varywith analyte
concentration if the column is not highly inert. Since the amount of target analyte in sam-
ples is unknown, retention times on a poorly deactivated column can easily vary enough to
move compounds outside the retention timewindow (Figure 2). This can result in inaccu-
rate identifications, theneed formanual integration, andadditional revieworanalysisbefore
results canbe reported.Using inert Rxi® columns ensures that compounds elutewith good
signal-to-noise ratios at expected retention times, regardless of analyte concentration.
High inertness
• Symmetricpeaks
• Strong response
• Accurate, low-level detection
Poor inertness
• Reduced sensitivity
• Misidentification
• Incorrect results
LowerDetectionLimits
withGround-Breaking
ColumnTechnology
Time
GC_EX01116
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