~.
RESTEK
Using Guard Columns and Retention Gaps in GC (Part 2)
G"'8nl o;I;IlUmnl Ind not.nliJn ,.pl
I~
uled
wldlly In
,n
d1rt1m1tognophy
{Gel.
"'.ny "'. ",. ....
Yll
dl'lbl~
undelllt:lmdlnlllhe dll'l'erenge between these two
prtldum, 8Ylln "'GUllh
th..... "
Illlnm"'nt d,"",nonct In Ipplltlltll;ln. In 1I,,1t 1 oT
til"
, rtlde
we reviewed
re~ntl'on
IIlpl, whim
mll""'.no
lIled I'orfu.Q!'1n1l the
, .. mp..
o:umpon.n~
whln tltr1:>ducfnll 11"'111
(Ilq...
ld lll~l.
dll'llld:Iy
onto the
column. In
eantl1llt.llu.rd
wlu........ uled
tll prDtl:d:
the In"I'f'\:I",1 column fnIm
cont.umn"lIOn . au.reI eu........ InCl ..
~nll\ln
Il'P' blOth ......
Jt
tie coupled W
tile
InllytQI
w lllmn, Ind
til.
connectIOn ...tnld
pot'lntlll point of
I'l,k,
A
1'1'"''
Ipprvld1
It'
til
Inte llnlte
t he
rti\'wntlll n glp dftd:ly
Inta the
.n.~l
colu
~b~ll .
8r
Ippl'flnll •
· n ll _ 1¢"
coltlnlil te l1l nolgg.,.
tn.
It.tlon,, 'Yphfte
wn be
d.po.~d
O"tv In. c.rtrol1 pert "the col",,", 1110""'1 • d...
d:.....
~d
Hd:lgn
It
tile
Mlllnnln!l.
COlumn
coupling. not ..
qund
.nd ...rn'-nln", IlliI...tty
.~I",.d .
"", ..., • •
rtI'.....
1iI....
1'II
Qllu"'"5 and
dll;cu.. th, I'll.
"iii_i'll
Qlllf!'llil
c.dlnolo~ .
U_oflll..
tdOc+ ,••
Ttl.
pulpo•• of ....lnlil lil....
1'II
QI"'''''''
I.
to pro c.et th. In.tytk:IIl
Cl>klmn
from Qlnt......tlon .1nce
tl>e
••mplot Ifl.t
II;
Inlnld"..d
II;
not
IlwIV-~.
AIIhouIiJh ttw but
ch
tGIiJ..
pfly
Is
"bt.~
with "..an"
••mplot., Ifl. p..ctltal'''''ltfon
I.
Ifllt ..
"",I. d• •
n-up
~d u
Imlz:ed . nd ....
u..
"dirty"
Ilmplotl I" Inlnld"..d ontlllfl.
miutM.
SIo"", I••
ClIn
_Ill
partlal"tli. ",•
.,.,CIlmpoMnt:I.
d.riwltil.tion ".IiI.na, Ieonil: ... id" ••, . tid., b
_.1
ttwn CDmp<IIundtl ClIn
"t.~
..
_ttl
tl>e
ltatlonlry ph•••nd
Ih.y
0011 ..
"".",*
~
..
p
tlon
~
...
Ulu • ..,.
til.
OIIi1.....
lIon
,,'CIllullWl
p"rfCln'l'llin..
iI ••
1000
pro... .
but
It will
""'~"'"
MOlt of th. ti",.
Ih.
l
uri\iI,••
CD>ft'&.Ilsta ..
~
l'Rt ....U r(I ) 01till
calu.....
l lI(l
h'
Qla... "II IlIiI
'eelion
ad"quatfl .apa...1Io1'1
Is
Ita...d . M. nv
u.....
O'\I)D"
ttl
I:I>nMlla o ua""
(IIlu.......
".,1I1
ottrlol ....lV!al
column. Suell a guard mlurm
ill
d••
lli....
t::ad . NI can
bIo
trio'rYnH
wll lln mntllmiolal-.J
'nd
_nWII Iy
I'llplilClld.
Dep"nd~o
ol'llh••
pplcall~
lJUIrd
mlurrna
II.... " lhwr.
of
l .... k
ulttfl'
_nlr. .Clf141
PIa.
dlll'fll'llni elloal
for
O".rd mlurml, • ;U.rd
mluI'M
can
CIlNIIt
on ly of
tl..ttMt8d GI/l""', ", I GIll b4I "
co'tad Clpllary.
Deild:JIRtfId
capillary
tubing:
DlIi1df'ti1t::ad l\lnd
dlciI
tubing
can IMo
jMll'CII....
d
by
tIHI
Il'lIItar,IIlId
t ll<lll •
d"nn"d IIngth ClI'I
b"
muplsd
In
Il'ont
oItha
'l'lIl'/tlClIIl
CIlIur!'ft.
U!lOIl
mm
_tlall, a .ectlan of tIHIou a rd
column IIl'llnlOvad. When
ttMl
wholfl
lIUiIrd
Is
"_mHo
a
II'"
gUlrd
m lullWl CllII
IMo
CIIu/llld .
llM
dlllldvantlg" of CllDlg
Pilrts
oil
oItha
IlUiIrd
ooIum
Is
tNll
tile
t»IullYl IMommu
. horta,
,II1Id
th .
IIIIV
,IIlI'lId
I'lltllntlDn tn.s.
How"var, If . """il r
lenglh
Is
I tw• ..,. altfhlmtIHI
i1 u.rd
(lllullYl,
t IMdl.lIiI"
In
retenten
nne
become.
very predlcbbtl:.
A
~.lllYIot:ed
lIArd
t»IullYl
1IO
ntlul ln
bllId
focul lnil.
It
th,,1n)IdIon II not
op~"
tIMtnJ
w.
be
iI
~
Ill'!'IMt ......
,tot t
or.
Nta ntlan i1./l.
Olfied~b.JbInfI'
I>#!
the
gull'll
~
nec'dI
to
ptC"i'ent
CIIIfU.-...t1on
of
the . ...
lVtlCllll
colu""','
coated i1Ullrd column
CIIl
help ..
1: ""
both
tile
-tl1lIl
tlndffttlon
' 1'111.110
tlH= Itltlan.,., ph..e
laver.
Ttl......
Ie.t.nd ......
t
_...mal .'V-usIng
mated
lI_rd
t»Iu
(DI'
"",mlu"",.)
II
tel
buy two
. n.lytlta l
lwmr..
One
sepII..
1ion
<DIu"",
.nd
the
mn d
an~
bt!
uSt!d
tel
.
....ted
O
rd
Iu""",.
R'tImtNs semnd
w~
o:uI: 2m.edlGn••
1Id
<IIUple ••
~dlDn
In
fro nt of
tlH=
.nelytbl
paralt>n ..........
We ClIn
sa
s..-ltl mnt.mlnatlan
.ffo!cI:!II
I"'.k
. h. peJ"'5JXIMe
and
thefl.e COIn
rI!fI'lII<2the
g '" wlh a ...... 2m ...dlDn.
The
system 00"
"-",,,~
_produce ...
pn;KllOdble
retention
tlmn ...e •••ys.1II rtl'pltCll! the entl",
2m ....ted II
5lna!
the
stIIttona,.,
phil ...
lithe
SI"'" on the IiIU.rd as on
th~
.n.1vt1Clll1 column,
th e "' •• " no
"'~
•.",.,
aNted
o;obon
.1s1I • •
110.
rnonl!
aggrtl'sl"'" Mmple.sllllllrtl'
...
nt.mlnellllln"~
1t • •
1iJ!We
up.lIsttyo
bIe
t o
o:uI:
15
t~d
gu.'" allumi'll from. rul 30m
. n.lytltal colurm... tlIIt"t
Mo
e_lOO,ktll
_<!!V<!!1'.
"us"'ll •
<11llt~d
gu.rd column. thenl!.1II be no
IocvsInliJ
~lIed5 .
• • ,."" CoMt'lt
1''''
I I. 111m.- P....lItNtle
CDlII=o:tIcN.
llIOth ..tentbn
9"PI
and .......
cot..mt!!'llSt
be
coupled t il t he . nalvtlc:lll column. While thenl!
"nI!
leYl!!n!Il
r,p..
O"~COII~
deYtotil,.1 ClI"
i7fl.t8
d".d
voktrntl
.nd can
bIi"
potantltl.soul'Cli of "'ales "nd
.._ ....,. 5efl\"lllnt ....
ti'\t
t8CtwIaIoiJ'
.lbwl
Iht
r8t"ntion g.p Or gu.'" co lumn
tel
bIi
buill dinl!dlv in th"
112
2012
3
