615
GCCHROMATOGRAMS |
FOODS, FLAVORS&FRAGRANCES
Fatty Acids
FattyAcids (Free)
Rtx®-200
1
2
3
4
5
6
7 8
9
min. 1
2
3
4
GC_CH00282
Column:
Rtx
®
-200, 30m, 0.25mm ID, 0.25µm (cat.# 15023)
Sample:
0.8µL split injection of a free fatty acid standard
Conc.:
approximately 10 to 20ng/µL
Oven temp.:
90°C
Inj./det. temp.:
250°C
Carrier gas:
hydrogen
Linear velocity:
40cm/sec. (flow rate: 1.4cc/min.)
FID sensitivity:
4 x 10
-11
AFS
Split vent:
40cc/min.
1. acetic acid
2. propionic acid
3. isobutyric acid
4.
n
-butyric acid
5. isovaleric acid
6.
n
-valeric acid
7. isocaproic acid
8. caproic acid
9. heptanoic acid
FattyAcids (Free)
Stabilwax®-DA
Column:
Stabilwax
®
-DA, 30m, 0.25mm ID, 0.25µm
(cat.# 11023)
Sample:
1.0µL split injection of a free acid standard
Conc.:
approximately 10 to 20ng/µL
Oven temp.:
145°C
Inj./det. temp.:
250°C
Carrier gas:
hydrogen
Linear velocity:
40cm/sec.
FID sensitivity:
2 x 10
-11
AFS
Split ratio:
50:1
GC_CH00280
min. 4
8
12
9
8
7
6
5
4
1
3
2
1. acetic acid
2. propionic acid
3. isobutyric acid
4.
n
-butyric acid
5. isovaleric acid
6.
n
-valeric acid
7. isocaproic acid
8. caproic acid
9. heptanoic acid
FattyAcids (Free)
Rtx®-1
GC_CH00281
min. 4
8
1
2
3 4
5
6
7 8 9
Column:
Rtx
®
-1, 30m, 0.53mm ID, 5.0µm (cat.# 10179)
Sample:
0.2µL injection of a 10–20ng/µL free fatty acid
standard inwater.
Inj.:
direct injection using aUniliner
®
inlet liner
Oven temp.:
60°C to 180°C@ 15°C/min.
Inj./det. temp.:
250°C
Carrier gas:
hydrogen
Linear velocity:
50cm/sec. (flow rate: 6cc/min.)
FID sensitivity:
4 x 10
-11
AFS
1. acetic acid
2. propionic acid
3. isobutyric acid
4.
n
-butyric acid
5. isovaleric acid
6.
n
-valeric acid
7. isocaproic acid
8. caproic acid
9. heptanoic acid
Noderivatization
needed!
Noderivatization
needed!
Noderivatization
needed!
RestekCustomer Service
In theU.S.
Call: 800-356-1688 (ext. 3) or 814-353-1300 (ext. 3)
Monday–Friday8:00a.m.–6:00p.m. ET
Fax: 814-353-1309—24-hoursaday
Online:www.restek.com—24-hoursaday
Outside theU.S.
Contact your Restek representative:
Refer toour listonpages4-5or visitourwebsiteat
PaulaZuchowski, Customer Service
Website :
E-mail :
TelNo : 03 9762 2034 . . . inAUSTRALIA
Mar 2011
