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Competitor Comparison: Class 2 - Mix A Residual Solvents for USP <467>Water-Soluble Articles
GC COLUMNS | APPLICATION-SPECIFIC COLUMNS
Pharmaceutical
Organic Volatile Impurities (OVI) Analysis
GC_PH1161
Column Rxi®-624Sil MS, 30 m, 0.32 mm ID, 1.80 µm (cat.# 13870)
Sample Residual solvents class 2 - mix A (cat.# 36271)
Diluent: Water
Injection Headspace-loop split (split ratio 5:1)
Liner: 1 mm split (cat.# 20972)
Headspace-Loop
Inj. Port Temp.: 140 °C
Instrument: Tekmar HT3
Inj. Time: 1 min
Transfer Line Temp.: 110 °C
Valve Oven Temp.: 110 °C
Sample Temp.: 80 °C
Sample Equil. Time: 60 min
Vial Pressure: 10 psi
Pressurize Time: 0.5 min
Pressure
Equilibration Time: 0.05 min
Loop Pressure: 5 psi
Loop Fill Time: 0.1 min
Oven
Oven Temp.: 40 °C (hold 20 min) to 240 °C at 10 °C/min (hold 20 min)
Carrier Gas He, constant flow
Linear Velocity: 35 cm/sec
Dead Time: 1.45 min @ 40 °C
Detector FID@ 250 °C
Data Rate: 5 Hz
Instrument Agilent/HP6890 GC
Acknowledgement Teledyne Tekmar
Peaks tR (min) Conc.
(µg/mL)
1. Methanol 2.281 25.00
2. Acetonitrile 4.009 3.42
3. Dichloromethane 4.313 5.00
4. trans-1,2-Dichloroethene 4.798 7.83
5. cis-1,2-Dichloroethene 7.028 7.83
6. Tetrahydrofuran 7.706 5.75
7. Cyclohexane 8.708 32.33
8. Methylcyclohexane 14.099 9.83
9. 1,4-Dioxane 15.054 3.17
10. Toluene 22.018 7.42
11. Chlorobenzene 26.570 3.00
12. Ethylbenzene 26.837 3.07
13. m-Xylene 27.147 10.85
14. p-Xylene 27.147 2.53
15. o-Xylene 27.927 1.63
Improve system suitability pass rates
with greater resolution on Rxi®-624Sil MS
columns.
Similar to: (6%-cyanopropylphenyl)-methylpolysiloxane
Rxi®-624Sil MS Columns (fused silica)
(midpolarity Crossbond® phase)
• Low-bleed, high-thermal stability column—maximum temperatures up to 320 °C.
• Inert—excellent peak shape for a wide range of compounds.
• Selective—G43 phase highly selective for volatile organics and residual solvents,
great choice for USP<467>.
• Manufactured for column-to-column reproducibility—well-suited for validated
methods.
Rxi®-624Sil MS Structure
x
Si O
(CH2) 3
CH3 CH3
CH3
Si O R O
CH3
CH3
Si
94% y
CH3
CH3
Si
C N
similar phases
DB-624, VF-624ms, CP-Select 624 CB
20-Meter 30-Meter 60-Meter 75-Meter 105-Meter
ID df temp. limits cat.# price cat.# price cat.# price cat.# price cat.# price
0.18 mm 1.00 µm -20 to 300/320 °C 13865
0.25 mm 1.40 µm -20 to 300/320 °C 13868 13869
0.32 mm 1.80 µm -20 to 300/320 °C 13870 13872
0.53 mm 3.00 µm -20 to 280/300 °C 13871 13873 13874 13875
G43 phase