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Over 10,000 Injections of Lipidomics on Rxi®-5ms
Rxi®-5ms Columns (fused silica)
(low-polarity phase; Crossbond® diphenyl dimethyl polysiloxane)
• General-purpose columns for semivolatiles, phenols, amines, residual solvents,
drugs of abuse, pesticides, PCB congeners (e.g., Aroclor mixes), solvent impurities.
• Most inert column on the market.
• Tested and guaranteed for ultra-low bleed; improved signal-to-noise ratio for better
sensitivity and mass spectral integrity.
• Temperature range: -60 °C to 330/350 °C.
• Equivalent to USP G27 and G36 phases.
Rxi®-5ms Structure
CH3
CH3
Si
95%
O Si
5%
O
Similar to: (5%-phenyl)-methylpolysiloxane
free literature
Rxi® Columns
Lower Detection Limits with Ground-
Breaking Column Technology
Download your .
free copy from
lit. cat.# .
GNFL1173A
GC_PH01053
Column Rxi®-5ms, 30 m, 0.25 mm ID, 0.25 µm (cat.# 13423)
Sample Lipid plasma extract as trimethylsilyl derivatives
Injection
Inj. Vol.: 1 µL split (split ratio 10:1)
Liner: 4 mm split liner gooseneck with wool
Inj. Temp.: 310 °C
Oven
Oven Temp.: 250 °C (hold 1 min) to 320 °C at 30 °C/min (hold 1.6 min)
Carrier Gas He, constant flow
Flow Rate: 1.0 mL/min
Detector MS
Mode: SIM
Transfer Line Temp.: 330 °C
Ionization Mode: EI
Peaks m/z
1. Coprostanol 370.40
2. d5-cholestanol (IS) 220.20
3. 7α-hydroxycholesterol 456.40
4. Cholestanol 306.30
5. d5-epicholestanol (IS) 220.20
6. 7-Dehydrocholesterol 325.25
7. Desmosterol 343.25
8. Lathosterol 458.35
9. d4-lathosterol (IS) 462.40
10. Campesterol 382.35
11. 4-Cholestenone 382.30
12. Stigmasterol 394.15
13. Lanosterol 393.35
14. β-Sitosterol 396.35
15. d7-β -sitosterol (IS) 403.45
GC COLUMNS | HIGH-PERFORMANCE Rxi® COLUMNS
Rxi®-5ms
15-Meter 30-Meter 60-Meter
ID df temp. limits cat.# price cat.# price cat.# price
0.25 mm 0.25 µm -60 to 330/350 °C 13420 13423 13426
0.40 µm -60 to 330/350 °C 13481
0.50 µm -60 to 330/350 °C 13435 13438 13441
1.00 µm -60 to 330/350 °C 13450 13453 13456
0.32 mm 0.25 µm -60 to 330/350 °C 13421 13424 13427
0.50 µm -60 to 330/350 °C 13436 13439 13442
1.00 µm -60 to 330/350 °C 13451 13454 13457
0.53 mm 0.25 µm -60 to 330/350 °C 13422 13425
0.50 µm -60 to 330/350 °C 13437 13440
1.00 µm -60 to 330/350 °C 13452 13455
1.50 µm -60 to 330/350 °C 13467 13470
12-Meter 20-Meter 25-Meter 50-Meter
ID df temp. limits cat.# price cat.# price cat.# price cat.# price
0.18 mm 0.18 µm -60 to 330/350 °C 13402
0.30 µm -60 to 330/350 °C 13409
0.36 µm -60 to 330/350 °C 13411
0.20 mm 0.33 µm -60 to 330/350 °C 13497 13498 13499
similar phases
HP-5ms SemiVolatiles, HP-5ms, HP-5msUI,
DB-5, Ultra-2, CP-Sil 8 CB, ZB-5, ZB-5msi
Stringent quality testing ensures consistent
performance, column to column and .
injection to injection.