83
FAMEs (
cis/trans
isomers) on Rt®-2560
GC COLUMNS |
APPLICATION-SPECIFIC COLUMNS
Foods, Flavors, & Fragrances
FAME Analysis (
cis
/
trans
)
32
33
34
35
1
2
4
5
3 6
7
8
36
37
38
39
40
41
42
GC_FF00652
Column
Rt®-2560, 100 m, 0.25 mm
ID, 0.20 µm (cat.# 13199)
Sample
cis
/
trans
FAME mix
(cat.# 35079)
Diluent:
Methylene chloride
Conc.:
10 mg/mL total FAMEs
Injection
Inj. Vol.:
1 µL split (split ratio 20:1)
Liner:
Splitless (4 mm ID)
(cat.# 20814)
Inj. Temp.:
225 °C
Oven
Oven Temp.:
100 °C (hold 4 min) to 240 °C
at 3 °C/min (hold 10 min)
Carrier Gas
H
2
, constant flow
Flow Rate:
1.2 mL/min
Detector
FID@ 250 °C
Peaks
Conc.
(wt.%)
1. C18:0 methyl stearate
20.0
2. C18:1 methyl petroselaidate (
trans
-6)
8.0
3. C18:1 methyl elaidate (
trans
-9)
10.0
4. C18:1 methyl transvaccenate (
trans
-11)
12.0
5. C18:1 methyl petroselinate (
cis
-6)
8.0
6. C18:1 methyl oleate (
cis
-9)
10.0
7. C18:1 methyl vaccenate (
cis
-11)
12.0
8. C18:2 methyl linoleate (
cis
-9,12)
20.0
Excellent resolution of
cis
&
trans
isomers!
FAMEs (Marine Oil Standard) on FAMEWAX
FAME Analysis (Polyunsaturated)
GC_FF00499
Column
FAMEWAX, 30 m, 0.32 mm ID, 0.25 µm
(cat.# 12498)
Sample
Marine oil FAME mix (cat.# 35066)
Diluent:
Isooctane
Conc.:
10,000 µg/mL (total FAMEs; see breakdown
in peak list)
Injection
Inj. Vol.:
1 µL split (split ratio 100:1)
Inj. Temp.:
250 °C
Oven
Oven Temp.:
195 °C to 240 °C at 5 °C/min (hold 1 min)
Carrier Gas
H
2
, constant flow
Flow Rate:
3 mL/min
Detector
FID@ 275 °C
Peaks
Conc.
(µg/mL)
1. C14:0
600
2. C14:1
100
3. C16:0
1,600
4. C16:1
500
5. C18:0
800
6. C18:1 (oleate)
1,300
7. C18:1 (vaccenate) 400
8. C18:2
200
9. C18:3
200
10. C20:0
100
11. C20:1
900
12. C20:2
100
13. C20:4
300
14. C20:3
100
15. C20:5
1,000
16. C22:0
100
17. C22:1
300
18. C24:0
100
19. C22:6
1,200
20. C24:1
100
Column engineered for
optimal resolution.
FAMEs Analyses
High-Resolution GC Analyses
of Fatty Acid
Methyl Esters
lit. cat.# 59584B
www.restek.comDownload your
free copy from
Rt®-2560 Column
(fused silica)
(highly polar phase; biscyanopropyl polysiloxane—not bonded)
• Application-specific column for
cis
/
trans
FAMEs.
• Stable to 250 °C.
Because the Rt®-2560 stationary phase is not bonded, it should not be solvent rinsed.
similar phases
HP-88, CP-Sil 88, SPB-2560
100-Meter
ID
df
temp. limits
cat.#
price
0.25 mm 0.20 µm
20 to 250 °C
13199
FAMEWAX Columns (USP G16)
(fused silica)
(polar phase; Crossbond® polyethylene glycol)
• Application-specific columns for FAMEs, specially tested with a FAME mixture.
• Temperature range: 20 °C to 250 °C.
The elution order of polyunsaturated FAMEs on FAMEWAX columns is comparable to
that on other Carbowax® columns, but baseline resolution is achieved in significantly
less time.
similar phases
Select FAME, Omegawax
30-Meter
ID
df
temp. limits
cat.#
price
0.25 mm 0.25 µm 20 to 240/250 °C
12497
0.32 mm 0.25 µm 20 to 240/250 °C
12498
0.53 mm 0.50 µm
20 to 250 °C
12499