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165
LC COLUMNS
Columns for UHPLC
Restek-
manufactured
silica
Column Characteristics:
particle size:
1.9 µm, 3 µm, or
5 µm, spherical
pore size:
140 Å
carbon load:
8%
end-cap:
yes
pH range:
2.5 to 8
temperature limit: 80 °C
USP phase:
L11
phase category:
phenyl
ligand type:
unique Biphenyl
Biphenyl
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USLC® Column Interaction Profile
(See
page 161for more information.)
Biphenyl
Si
CH
3
CH
3
O
Biphenyl
Si
CH
3
CH
3
O
Chromatographic Properties
Since 2005, the Restek® Biphenyl has offered a greater degree of dispersion than conventional
phenyls and a greater degree of polarizability than phenyl hexyls, creating higher selectivity and
a greater range of usability. Because of these heightened interactions, this column shows sub-
stantial increases in retention—especially for dipolar, unsaturated, or conjugated solutes—and
enhanced orthogonal selectivity when using methanol mobile phases. It is ideal for increasing
sensitivity and selectivity in LC-MS analyses.
Pinnacle® DB Biphenyl Columns
(USP L11)
NSAIDs on Pinnacle® DB Biphenyl
LC_PH0478A
Column
Pinnacle® DB Biphenyl (cat.# 9409565)
Dimensions:
150 mm x 4.6 mm ID
Particle Size:
5 µm
Pore Size:
140 Å
Temp.:
30 °C
Sample
Diluent:
0.1% formic acid in water:methanol (40:60)
Conc.:
100 µg/mL each component (see peak list)
Inj. Vol.:
10 µL
Mobile Phase
A:
0.1% formic acid in water
B:
methanol
Time (min) %B
0.00
60
2.0
60
8.0
90
20.0
90
20.1
60
Flow:
1.0 mL/min
Detector
UV/Vis @ 254 nm
Instrument
Shimadzu Prominence
Peaks
1. Uracil (void marker)
2. Tenoxicam
3. Sulfinpyrazone
2.1 mm ID
3.0 mm ID
4.6 mm ID
Length
cat.#
price
cat.#
price
cat.#
price
1.9 µm Columns
30 mm
9409232
—
—
50 mm
9409252
—
—
100 mm
9409212
—
—
3 µm Columns
30 mm
9409332
940933E
9409335
50 mm
9409352
940935E
9409355
100 mm
9409312
940931E
9409315
150 mm
9409362
940936E
9409365
5 µm Columns
30 mm
9409532
940953E
9409535
50 mm
9409552
940955E
9409555
100 mm
9409512
940951E
9409515
150 mm
9409562
940956E
9409565
200 mm
9409522
940952E
9409525
250 mm
9409572
940957E
9409575
Time (min)