Sample:
Inj.:
5µL
Conc.:
30µg/mL
(30ppm) each
Sample diluent: acetonitrile
Column:
Ultra C18
Cat.#:
9174575
Dimensions:
250 x 4.6mm
Particle size:
5µm
Pore size:
100Å
Conditions:
Mobile phase:
water:methanol
(44:56, v/v)
Flow:
1.0mL/min.
Temp.:
30°C
Det.:
UV@ 254nm
ExplosivesbyHPLC
UltraC18Columns (USPL1)
PhysicalCharacteristics:
5µmColumn, 4.6mm
cat.#
price
150mm
9174565
$372
250mm
9174575
$427
particle size: 3µmor
5µm, spherical
pore size: 100Å
carbon load: 20%
endcap: fully endcapped
pH range: 2.5 to7.5
temperature limit: 80°C
Pinnacle™ IIBiphenylColumns (USPL11)
PhysicalCharacteristics:
5µmColumn, 4.6mm
cat.#
price
150mm
9209565
$360
250mm
9209575
$412
ordering note
For guard cartridges for these columns, visit ourwebsite at
particle size: 5µm,
spherical
pore size: 110Å
endcap: yes
pH range: 2.5 to7.5
temperature limit: 80°C
1,3-dinitrobenzene
2-amino-4,6-dinitrotoluene
4-amino-2,6-dinitrotoluene
2,4-dinitrotoluene
2,6-dinitrotoluene
HMX
nitrobenzene
2-nitrotoluene
3-nitrotoluene
4-nitrotoluene
RDX
tetryl
1,3,5-trinitrobenzene
2,4,6-trinitrotoluene
NitroaromaticsandNitramine
ExplosivesbyHPLC
(14 components)
1,000µg/mL each in acetonitrile, 1mL/ampul
cat.# 33905 (ea.)
$95
3,4-dinitrotoluene
8095 InternalStandard
1,000µg/mL inmethanol, 1mL/ampul
cat.# 31452 (ea.)
$23
1,2-dinitrobenzene
8330Surrogate
1,000µg/mL inmethanol, 1mL/ampul
cat.# 31453 (ea.)
$23
Commonmethods for quantifying explosives call for reversed phase HPLC
with UV detection, using a primary column and a confirmation column.
While cyano phases typically have been used for the confirmation column,
resolution of the target explosive compounds is poor. The Pinnacle™ II
Biphenyl columnprovides excellent resolutionofMethod 8330 explosives, as
shown inFigure1,and selectivity ismarkedlydifferent fromC18phases,mak-
ing the Pinnacle™ II Biphenyl column an ideal confirmation column. If a
cyano phasemust be used for confirmation, we recommend a Pinnacle™ II
Cyano column.
Figure1
Anoutstanding columnpair for explosivesanalysis.
0
2
4
6
8
10
12
14
16
18 min.
16
15
14
13
11
9
12 10
6
8
7
5
4
3
2
1
LC_0254
LC_0322
Peak List:
1. HMX
2. RDX
3. 1,2-dinitrobenzene (SS)
4. 1,3,5-trinitrobenzene
5. 1,3-dinitrobenzene
6. tetryl
7. nitrobenzene
8. 3,4-dinitrotoluene (IS)
9. 2,4,6-trinitrotoluene
10. 4-amino-2,6-dinitrotoluene
11. 2-amino-4,6-dinitrotoluene
12. 2,6-dinitrotoluene
13. 2,4-dinitrotoluene
14. 2-nitrotoluene
15. 4-nitrotoluene
16. 3-nitrotoluene
Sample:
Inj.:
10µL
Conc.:
30µg/mL (30ppm) each
Sample diluent: acetonitrile
Sample temp.: ambient
Standard:
Cat.#: 31452, 31453, 31450, 31451
Column:
Pinnacle
™
II Biphenyl
Cat.#:
9209565
Dimensions:
150 x 4.6mm
Particle size:
5µm
Pore size:
110Å
Conditions:
Mobile phase:
water:methanol, 0:50, v/v
Flow:
1.5mL/min.
Temp.:
ambient
Det.:
UV@ 254nm
...coupledwithaPinnacle II™Biphenyl column.
Forsuperiorperformance,useanUltraC18primarycolumn...
RecommendedColumns
AnalyticalReferenceMaterials
13
free
literature
HPLCAnalysis of Trace-Level ExplosivesUsing
Pinnacle II
™
C18 andCyanoColumns
Pinnacle II
™
C18 reversed phase columns andPinnacle II
™
Cyano
normal phase columns are effective primary and confirmation
columns for analyzing explosives according toUS EPAMethod
8330A. Analytical conditions and example chromatograms are
presented in this 2-page note. Per recommendation in the
method, the samemobile phase is used for the primary and
confirmation analysis.
Lit. cat.# 59361
