○
○
○
○
○
○
○
○
○
○
○
○
○
○
○
○
5
Figure 3
The Rt-
β
DEXsm column provides
the best chiral separation of
isoborneol and
α
-terpineol.
Figure 4
The Rt-
β
DEXsm column offers
complete resolution of
α
-ionone.
30m, 0.32mm ID, 0.25µmRt-
β
DEXsm (cat.# 13104)
Oven temp.:
40°C (hold 1min.) to 230°C@ 2°C/min. (hold 3min.);
Carrier gas:
hydrogen; 80cm/sec. set @ 40°C;
Detector:
FID set @ 220°C
min. 30
40
(+/-) isoborneol
(+/-) terpineol
min. 30
40
α
-ionone
Figure 6
The Rt-
β
DEXse column resolves
limonene enantiomers.
min. 18
20
limonene
Figure 5
The Rt-
β
DEXse column resolves optical isomers of
ethyl-2-methylbutyrate, styrene oxide, and camphor
with some overlap.
min. 10
20
30
ethyl-2-methylbutyrate
styrene oxide
camphor
R S
S (-)
R (+)
30m, 0.32mm ID, 0.25µm Rt-
β
DEXse
(cat.# 13106)
Oven temp.:
40°C (hold 1min.) to 230°C@
2°C/min. (hold 3min.);
Carrier gas:
hydrogen;
80cm/sec. set @ 40°C;
Detector:
FID set @ 220°C
RESTEK’S CHIRAL COLUMNS OFFERUNIQUE
SELECTIVITY
○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○
Each of the five chiral columns
posesses a specific combination of
alkyl substituents on the derivatized
β
-cyclodextrins. Theseuniquecombi-
nations provide a wide range of
utilitization for each type of chiral
column. Table I, on page 8, indicates
that certain columns provide better
resolution of specific compounds.
Rt-
β
DEXsm
○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○
Of thechiral columnsevaluated, only
theRt-
β
DEXsmseparatesallof the25
tested compounds, with 19 being
baseline resolved. This column pro-
vides the best enantiomeric separa-
tionof
α
-pinene, isoborneol,
α
-ionone,
linalool oxides,hexobarbital, andme-
phobarbital (
Figures 3 and 4
).
Rt-
β
DEXse
○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○
The Rt-
β
DEXse is similar in perfor-
mance to theRt-
β
DEXsm, but it pro-
vides better resolution for limonene,
linalool, linalyl acetate, ethyl-2-
methylbutyrate, 2,3-butane-diol, and
styrene oxides. Sometimes extensive
separation results inoverlapof enan-
tiomericpairs, asshown in
Figures5
and 6
.
SR
