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Ciprofloxacin
Lomefloxacin
Sparfloxacin
Preliminary Separation
Mobile Phase: A = 10mM Potassium
Phosphate, pH 2.5 : B = ACN
Flow rate: 1.0 mL/min.
UV detection @ 220nm
Sample Volume: 5µl of 50µg/mL
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Goal of Analysis:
•
High throughput - fast and
selective
Levofloxacin
Retention and Selectivity of Fluoroquinolones
Enrofloxacin
Norfloxacin
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