Basic HTML Version
2,5-dihydroxy
benzoic acid
3,5-dihydroxy
benzoic acid
2,4-dihydroxy
benzoic acid
benzoic acid
4-hydroxy
benzoic acid
3-hydroxy
benzoic acid
salicylic acid
Mobile Phase A=20mM K
x
(PO
4
)
y
, pH 2.5
Mobile Phase B=ACN
Flow rate: 1.0 mL/min, gradient
UV detection @ 210 nm
Temperature: Ambient
Sample Volume: 5µL of 50µg/mL
Time %A
%B
0
80
20
5
50
50
10
50
50
Preliminary Separation
1
2
3
4
5
6
7
Example: Hydroxybenzoic Acids
13
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