Page 35 - Tubing-Chromatography_1-61

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Tubing for chromatography
High pressure fittings
• Nanovolume
• Stainless steel
• Polymeric
Low pressure fittings
Filters & mobile phase filters
Mobile phase handling systems
Frits
PEEK columns & guard columns
Sample loops
Accessories/miscellaneous
Knitted PTFE Reaction Coils
l
Tortuous flow path of optimal flow dynamic design
l
Made of inert PTFE
l
Minimized axial dispersion combined with efficient radial mixing
l
1/16" OD tubing in a variety of lengths and IDs
Preserved chromotographic peak shape
The Knitted PTFE Reaction coils (KRC) are made from tubing
which has been knitted into a tortuous path to force the fast moving
centre of the liquid stream to mix radially with the slower moving
boundary liquid layer, thereby minimizing the axial dispersion. The
shape of a chromatographic peak entering the reactor is thus
preserved very efficiently. The KRC is usually the most optimal
delay element for use in analytical flow systems. A coil is typically
inserted in the flow path to create a delay line, so that a reaction or
other event that requires a certain time can take place. Different
delay or reaction times are accomplished by changing the inner
diameter and length of the KRC, taking the flow rate through the
KRC into consideration.
Note:
To ensure a stable radial mixing within the KRC, a linear
flow rate of 10 cm/sec or more is recommended.
Example application areas
KRC can be applied as a delay line in chromatographic separations. One example is
the parallel coupling of a MS detector and a fraction collector in preparative
separations (see
illustration
)
. The delay time induced by the KRC allows time for
detection and therefore an intelligent decision between the fraction collection or
waste without loss of chromatographic efficiency. The KRC reactors can also be
used in post-column reaction detection in HPLC (see
illustration
)
. In this set-up the
KRC also ensures thorough mixing between the column effluent and the added
reagents.
1/16" Knitted PTFE Reaction Coils
Tolerances: ID and OD: ± 0.05 mm (.002")
Pressure rating: <55 bar (<800 psi)
96
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