0
2
4
6
8
Retention time/min
Column: SunShell C18, 2.6
µ
m 100 x 2.1 mm
Mobile phase: CH
3
CN/H
2
O=60/40
Flow rate: 0.3 mL/min
Pressure: 12.5 MPa
Temperature: 25 ºC
Sample: 1 = Uracil
2 = Toluene
3 = Acenaphthene
4 = Butylbenzene
Plate(3)=21,300
1
2
3
4
Column: SunShell C18, 2.6
µ
m 150 x 4.6 mm
1
Column: SunShell C18, 100 x 2.1 mm
Column: SunShell C18,150 x 4.6 mm
Column: SunShell C18, 50 x 2.1 mm
0
1
2
3
4
5
Plate(3)=10,300
Column: SunShell C18, 2.6
µ
m 50 x 2.1 mm
Mobile phase: CH
3
CN/H
2
O=60/40
Flow rate: 0.3 mL/min
Pressure: 7 MPa
Temperature: 23 ºC
1
2
3
4
UHPLC
Retention time/min
Efficiency of SunShell C18
Efficiency=253,000 plate/m
0
2
4
6
8
10
0
2
4
6
8
10
1
2
3
4
Retention time/min
Column: SunShell C18, 2.6
µ
m 150 x 4.6 mm
SunShell C18, 2.6
µ
m 100 x 4.6 mm
Mobile phase: CH
3
CN/H
2
O=70/30
Flow rate: 1.0 mL/min
Pressure: 14.5MPa(UHPLC),13.5 MPa(HPLC) for 150 mm
9.5MPa
（
HPLC) for 100 mm
Temperature: 25 ºC
Sample: 1 = Uracil
2 = Toluene
3 = Acenaphthene
4 = Butylbenzene
0
2
4
6
8
10
2
3
4
Retention time/min
Plate(3)=38,000
Retention time/min
HPLC
80% efficiency to
compare with UHPLC
Column: SunShell C18, 150 x 4.6 mm
Column: SunShell C18, 100 x 4.6 mm
Plate(3)=20,000
Plate(3)=31,000
3
4
2 1
The same efficiency as
5
µ
m, 250 x 4.6 mm
Saving 60% for
analytical time and
consumption of solvent
LaChrom ELITE
Website : www.chromtech.net.au E-mail : info@chromtech.net.au TelNo : 03 9762 2034 . . . in AUSTRALIA