◆
Evaluation of Stability
Evaluation of Stability
Accelerated acidic test
Durable test condition
Column size: 50 x 2.1 mm
Mobile phase: CH
3
CN/1.0% TFA, pH1=10/90
Flow rate: 0.4 mL/min
Temperature: 80 ºC
Measurement condition
Column size: 50 x 2.1 mm
Mobile phase: CH CN/H O=60/40
Durable test condition
Column Size: 50 x 2.1 mm
Mobile phase:
CH
3
OH/20mM Sodium borate/10mM NaOH=30/21/49 (pH10)
Flow rate: 0.4 mL/min
Temperature: 50 ºC
Alkaline test
1% TFA
80 ºC
pH10
50 ºC
Mobile phase: CH
3
CN/H
2
O=60/40
Flow rate: 0.4 mL/min
Temperature: 40 ºC
Sample: 1 = Uracil (t
0
)
2 = Butylbenzene
Temperature: 50 ºC
Measurement condition
Column Size: 50 x 2.1 mm
Mobile phase: CH
3
CN/H
2
O=60/40
Flow rate: 0.4 mL/min
Temperature: 40 ºC
Sample: 1 = Butylbenzene
Stability under acidic pH condition was evaluated at 80 ºC using
acetonitrile/1% trifluoroacetic acid solution (10:90) as mobile
phase. 100% aqueous mobile phase expels from the pore of
packing materials by capillarity and packing materials doesn’t
deteriorate. 10% acetonitrile in a mobile phase allows an
accurate evaluation.
1-3)
★
Sunshell C18 has kept 90% retention for 100
hours under such a severe condition. SunShell
C18 is 5 to 10 times more stable than the other
core shell C18.
Stability under basic pH condition was evaluated at 50 ºC using
methanol/Sodium borate buffer pH 10 (30:70) as mobile phase.
Sodium borate is used as a alkaline standard solution for pH
meter, so that its buffer capacity is high.
Elevated temperature of 10 ºC makes column life be one third.
The other company shows stability test at ambient (room
temperature). If room temperature is 25 ºC, column life at room
temperature (25 ºC) is sixteen times longer than that at 50 ºC.
★
SunShell C18 is enough stable even if it is used
under pH 10 condition. Regarding stability under
basic pH condition, there is little C18 column like
SunShell C18 except for hybrid type C18. It is
considered that our end-capping technique leads
high stability.
★
SunShell C18 can be used at the pH
range from 1.5 to 10.
1) N. Nagae, T. Enami and S. Doshi, LC/GC North America October 2002.
２
) T. Enami and N. Nagae, American Laboratory October 2004.
３
) T. Enami and N. Nagae, BUNSEKI KAGAKU, 53 (2004) 1309.
Website : www.chromtech.net.au E-mail : info@chromtech.net.au TelNo : 03 9762 2034 . . . in AUSTRALIA