Characteristics of SunShell C18
Core shell silica
C18
Particle size
(
µ
m)
Pore diameter
(nm)
Specific surface area
(m
2
/g)
Carbon content
(%)
Bonded
phase
Maximum operating
pressure
Available pH
range
SunShell C18
2.6
9
150
7
C18
60 MPa or 8,570 psi
1.5 - 10
0 2 4 6 8 10 12 14 16 18 20 22 24
Retention time/min
Column:
Brand D C18, 2.6
µ
m 150 x 4.6 mm (Core shell type)
Brand E C18, 2.7
µ
m 150 x 4.6 mm (Core shell type)
SunShell C18, 2.6
µ
m 150 x 4.6 mm
Mobile phase: CH
3
OH/H
2
O=75/25
Flow rate: 1.0 mL/min
Temperature: 40
º
C
Sample: 1 = Uracil, 2 = Caffeine, 3 = Phenol, 4 = Butylbenzene
5 = o-Terphenyl, 6 = Amylbenzene, 7 = Triphenylene
Comparison of standard samples between core shell C18s
Comparison of pyridine
Comparison of Oxine
Comparison of formic acid
Brand D C18 (Core shell)
Brand E C18 (Core shell)
SunShell C18 (core shell)
Retention of standard samples was compared for three kinds of core shell type C18s. Brand D C18 showed only a half retention to
compare with SunShell C18. Steric selectivity becomes large when ligand density on the surface is high. SunShell C18 has the
largest steric selectivity so that it has the highest ligand density. This leads the longest retention time.
Hydrogen bonding
(Caffeine/Phenol)
Hydrophobicity
(Amylbenzene/Butylbenzene)
Steric selectivity
(Triphenylene/o-Terphenyl)
Brand D C18
0.48
1.54
1.20
Brand E C18
0.44
1.60
1.31
SunShell C18
0.40
1.59
1.47
1
1
1
2
2
2
7
3
3
3
4
4
4
5
5
5
6
6
6
7
7
0 1 2 3 4 5 6 7
8 9 10101010 11111111 12121212 13131313
Retention time/min
Column:
Brand D C18, 2.6
µ
m 150 x 4.6 mm
Brand E C18, 2.7
µ
m 150 x 4.6 mm
SunShell C18, 2.6
µ
m 150 x 4.6 mm
Mobile phase: CH
3
OH/H
2
O=30/70
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Detection: UV@250nm
Sample: 1 = Uracil
2 = Pyridine
3 = Phenol
Brand D C18
(Core shell)
Brand E C18
(Core shell)
SunShell C18
(Core Shell)
1
1
2
3
2
3
1
2
3
0 1 2 3 4 5 6
7 8 9 10101010
Retention time/min
Column:
Brand D C18, 2.6
µ
m 150 x 4.6 mm
Brand E C18, 2.7
µ
m 150 x 4.6 mm
SunShell C18, 2.6
µ
m 150 x 4.6 mm
Mobile phase: CH
3
CN/20mMH
3
PO
4
=10/90
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Detection: UV@250nm
Sample: 1 = 8-Quinolinol (Oxine)
2 = Caffeine
Brand D C18
(Core shell)
Brand E C18
(Core shell)
SunShell C18
(Core shell)
2
1
1
2
2
1
Brand D C18
(Core shell)
Brand E C18
(Core shell)
SunShell C18
(Core shell)
0 1 2 3 4 5
6
Retention time/min
Column:
Brand D C18, 2.6
µ
m 150 x 4.6 mm
Brand E C18, 2.7
µ
m 150 x 4.6 mm
SunShell C18, 2.6
µ
m 150 x 4.6 mm
Mobile phase: CH
3
CN/0.1% H
3
PO
4
=2/98
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Detection: UV@210nm
Sample: 1 = Formic acid
2 = Acetic acid
3 = Propionic Acid
1
1
2
2
1
2
3
3
3
Residual silanol groups make pyridine be
tailing under methanol/water mobile phase
condition. Only SunShell C18 shows a
sharp peak for pyridine.
8-Quinolinol (Oxine) is a metal chelating
compound. Metal impurities in the core
shell particle leads the tailing for oxine
peak.
Formic acid is used as an indicator for a
acidic inertness. SunShell and brand D
C18 show a sharp peak.
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