Transfer Guide
from conventional column to SunShell (core-shell) column
1) Choice of column dimension. Terms: same efficiency, same theoretical plate (TP)
In case of using HPLC
C18 5
m
m 250 x 4.6 mm, 20,000 plate
SunShell C18 2.6
m
m 100 x 4.6 mm, 25,000 plate (UHPC)
20,000 plate (HPLC)
C18 5
m
m 150 x 4.6 mm, 12,000 plate
SunShell C18 2.6
m
m 75 x 4.6 mm, 15,000 plate (UHPC)
12,000 plate (HPLC)
SunShell C18 2.6
m
m 50 x 4.6 mm, 12,500 plate (UHPC)
10,000 plate (HPLC)
C18 3
m
m 150 x 4.6 mm, 20,000 plate
SunShell C18 2.6
m
m 100 x 4.6 mm, 25,000 plate (UHPC)
20,000 plate (HPLC)
C18 3
m
m 100 x 4.6 mm, 13,000 plate
SunShell C18 2.6
m
m 75 x 4.6 mm, 15,000 plate (UHPC)
12,000 plate (HPLC)
SunShell C18 2.6
m
m 50 x 4.6 mm, 12,500 plate (UHPC)
10,000 plate (HPLC)
In case of using UHPLC
C18 5
m
m 250 x 4.6 mm, 20,000 plate
SunShell C18 2.6
m
m 100 x 3.0 mm, 20,000 plate
SunShell C18 2.6
m
m 100 x 2.1 mm, 20,000 plate
C18 5
m
m 150 x 4.6 mm, 12,000 plate
SunShell C18 2.6
m
m 75 x 3.0 mm, 12,000 plate
SunShell C18 2.6
m
m 75 x 2.1 mm, 12,000 plate
SunShell C18 2.6
m
m 50 x 3.0 mm, 10,0000 plate
SunShell C18 2.6
m
m 50 x 3.0 mm, 10,0000 plate
SunShell C18 2.6
m
m 50 x 3.0 mm, 10,0000 plate
C18 3
m
m 150 x 4.6 mm, 20,000 plate
SunShell C18 2.6
m
m 100 x 3.0 mm, 20,000 plate
SunShell C18 2.6
m
m 100 x 2.1 mm, 20,000 plate
C18 3
m
m 100 x 4.6 mm, 13,000 plate
SunShell C18 2.6
m
m 75 x 3.0 mm, 12,000 plate
SunShell C18 2.6
m
m 75 x 2.1 mm, 12,000 plate
SunShell C18 2.6
m
m 50 x 3.0 mm, 10,0000 plate
SunShell C18 2.6
m
m 50 x 3.0 mm, 10,0000 plate
2) Decision of flow rate, injection volume and gradient time program.
Terms in case of 1.0 of coefficient. (More than 1.5 of coefficient is available for all conditions.)
Flow rate
SunShell
= Flow rate
3 or 5
m
m
x
x Coefficient (1.0 – 2.5)
Diameter
SunShell
Diameter
3 or 5
m
m
2
Injection volume
SunShell
= Injection volume
3 or 5
m
m
x
x
Length
SunShell
Length
3 or 5
m
m
Diameter
SunShell
Diameter
3 or 5
m
m
2
Gradient time
SunShell
= Gradient time
3 or 5
m
m
x
x
x
Flow rate
3 or 5
m
m
Flow rate
SunShell
Length
SunShell
Length
3 or 5
m
m
Diameter
SunShell
Diameter
3 or 5
m
m
2
Molecular weight of sample
Less than 400
More than 400
Mobile phase Methanol/water Acetonitrile/water Methanol/water Acetonitrile/water
Temperature
25
o
C
OK
OK
OK
OK
40
o
C
OK
Avoidance
OK
OK
* HPLC system time lag of beginning gradient elution should be considered.