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10
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SPE-05
1.3 Direct sample injection
SPE-05 can work as an auto sampler. Therefore, the HPLC does not need to have another sample
introduction device. This feature can reduce the cost of an HPLC system considerably.
2. Working principle
The following diagrams describe the structure and typical working procedures of the 3-in-1 model.
The plunger for SPE column can seal the column well. It can work with SPE columns from most suppliers.
There is no need for a special cap or adapter.
3. Application example
Direct analysis of hormone in plasma sample:
1)
Dilute plasma sample with 1% phosphoric acid at 1:1 ratio
2)
Precondition a 3-mL/500-mg C18 SPE column with 2 mL methanol followed by 2 mL water
3)
Load 2 mL sample and wash with 4 mL water+methanol (80:20)
4)
Wash SPE column using methanol and collect 1 mL fraction
Dansyl chloride
Estrone
60
℃
/pH10
+