Basic HTML Version
www.restek.com
69
GC COLUMNS |
APPLICATION-SPECIFIC COLUMNS
Foods, Flavors & Fragrances
GC_FF00652
FAMEs (
cis
/
trans
isomers) on an Rt®-2560 column.
Column:
Rt
®
-2560, 100m, 0.25mm ID, 0.2µm (cat.# 13199)
Sample:
cis
/
trans
FAME Mix (cat.# 35079), 10mg/mL total FAMEs in methylene chloride
Inj.:
1.0µL split (split ratio 20:1), 4mm inlet liner (cat.# 20814)
Inj. temp.:
225°C
Carrier gas:
hydrogen, constant flow
Flow rate:
1.2mL/min.
Oven temp.:
100°C (4 min. hold) to 240°C @ 3°C/min. (10 min. hold)
Det.:
FID @ 250°C
Compound
% in Mix
1. C18:0 methyl stearate
20.0
2. C18:1 methyl petroselaidate (
trans
-6)
8.0
3. C18:1 methyl elaidate (
trans
-9)
10.0
4. C18:1 methyl transvaccenate (
trans
-11)
12.0
5. C18:1 methyl petroselinate (
cis
-6)
8.0
6. C18:1 methyl oleate (
cis
-9)
10.0
7. C18:1 methyl vaccenate (
cis
-11)
12.0
8. C18:2 methyl linoleate (
cis
-9,12)
20.0
Excellent resolution
of
cis
&
trans
isomers!
cis
/
trans
FAMEs
similar
phases
SPB-2560, HP-88, Silar 10C, CP-Sil 88 FAME,
CP-Sil 88
Rt®-2560 Column
(fused silica)
(highly polar phase; biscyanopropyl polysiloxane—not bonded)
• Application-specific column for
cis
/
trans
FAMEs.
• Stable to 250 °C.
Because the Rt®-2560 stationary phase is not bonded, it should not be solvent rinsed.
Solutions For Your Foods, Flavors & Fragrances Analyses
Visit us at
www.restek.com/fff
Improved best-in-class GC columns • Standards • Industry experts at your service.
ID
df
temp. limits
100-Meter
0.25mm 0.20µm 20 to 250°C 13199
Website : www.chromtech.net.au E-mail : info@chromtech.net.au TelNo : 03 9762 2034 . . . in AUSTRALIA
Mar 2011