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In reversed phase and adsorption chromatography, the possibilities for using glass columns are often limited due to high
back pressures generated by small particles. The high pressure resistance of the Econoline® glass columns allow you to
achieve high flow rates for demanding separations. The example shows that this enables a considerable acceleration of
the separation, which means that you can achieve significant time savings.
30 ml/min
9 bar
N=2245
A
S
=1,32
75 ml/min
28 bar
N=1490
A
S
=1,19
125 ml/min
50 bar
N=1101
A
S
=1,22
Sample:
Uracil
0,175 mg/ml
Methylbenzoate 1,25 µl/ml
Naphthalene 0,5 mg/ml
Butyl benzoate 4,0 µl/ml
75% Time saving due to
high pressure resistance
= higher flows possible
Column:
ELS Econoline® 125 x 25 mm ID
Stationary phase: YMC-Pack ODS-AQ, 10 µm, 12 nm, 8 cm bed length
Flow rate:
30 ml/min - 75 ml/min - 125 ml/min
Mobile phase: ACN/H
2
O (50:50)
Injection:
500 µl
Detection:
UV at 254 nm
Separation of a standard test mixture of proteins
Column:
ELS Econoline® 250 x 15 mm ID
Stationary phase: YMC-BioPro SP, 30 μm (bed length 170 mm)
Mobile phase:
A) 20 mM KH
2
PO
4
*K
2
HPO
4
(pH 6.8)
B) 20 mM KH
2
PO
4
*K
2
HPO
4
(pH 6.8) containing 0.5 M NaCl
Gradient:
40-80% B
Flow rate:
6 ml/min
Temperature:
25°C
Detection:
UV at 220 nm
Injection:
100 μl
Sample:
1. Ribonuclease A
2. Cytochrome C
3. Lysozyme
Example of application with reversed phase media
Application Examples
Econoline
®
Series Glass Columns