Basic HTML Version
0 5 10 15 20 25 30 35 40 45
50 55
1
2
3
4
5
Retention time/min
Oxalic acid
Theanine
Guanylic acid
Column: Sunniest RP-AQUA , 5
μ
m 250 x 4.6 mm
Mobile phase: A) H
2
O/H
3
PO
4
(99.9:0.1)
B) CH
3
CN
Time (min) 0 20 60
%B (%) 0 0 50
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Detection: UV@210 nm
Injection volume: 10
μ
L
Sample: Oolong tea 1 = (-)-Epigallocatechin
2 = (+)-Catechin
3 = (-)-Epigallocatechin gallate
4 = (-)-Epicatechin
5 = (-)-Epicatechin gallate
Caffeine
◆
separation of Oolong tea
◆
Separation of water-soluble vitamins
Column: Sunniest RP-AQUA, 5
μ
m 150 x 4.6 mm
Mobile phase: 40mM Phosphate buffer pH6.8
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Detection: UV@250nm
Sample: 1 = Nicotinic acid, 2 = Pyridoxal
3 = Pyridoxine, 4 = Nicotinamide
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14
Retention time/min
1
2
3
4
◆
Separation of nucleotides
Column: Sunniest RP-AQUA, 3
μ
m 150 x 4.6 mm
Mobile phase: 20mM Phosphate buffer pH6.0
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Detection: UV@250nm
Sample: 1 = 5’-GDP, 2 = 5’-GMP, 3 = 5’-ATP, 4 = 5’-ADP,
5 = 5’-AMP
1 2
3
4
5
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14
Retention time/min
N=19,800
Sunniest C18
Sunniest RP-AQUA
Sunniest C8
0 2 4 6 8 10 12 14 16 18 20 22 24 26
Retention time/min
Column: Sunniest RP-AQUA, 5
μ
m 150 x 4.6 mm
Mobile phase: A) 20mM Ammonium acetate
B) Acetonitrile/ A solution (20:80)
Time (min) 0 5 20 25
%B (%) 0 0 100 100
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Detection: UV@250 nm
Sample: 1= Nicotinic acid
2 = Pyridoxine HCl Vitamin B
6
3 = Nicotinamide
4 = Thiamine HCl Vitamin B
1
5 = Folic acid
6 = Riboflavin Vitamin B
2
◆
Separation of water-soluble vitamins using
mobile phase for LC/MS
3
1
2
4
5
6
Website : www.chromtech.net.au E-mail : info@chromtech.net.au telNo : 03 9762 2034 . . . in AUSTRALIA