Basic HTML Version
■
Relationship between buffer concentration and
retention
(
Sunrise C18-SAC
)
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
0.5
1.0
1.5
2.0
2.5
3.0
log [buffer concentration]
log k
0
2,000
4,000
6,000
8,000
10,000
12,000
14,000
N [amitriptyline]
nortriptyline
amitriptyline
propranolol
toluene
N
(amitriptyline)
log
log[ ]
log[ ] log
log
k z
z
A z
z
A V
V z
K
B
A
B
A
r
m A
A
B
= −
+
+
+
1
A
：
ion in eluent
，
B
：
ion of sample
，
k
：
retention of
B
，
K
：
selection coefficient
ZA
,
Z
B
：
charge
，
Vr
,
Vm
：
resin volume and mobile phase volume in column
✻
Silanol groups controlled its activity functions as ion-exchange groups
■
Separation of basic compounds with ammonium
acetate: Effect of salt concentration
(
Sunrise C18-SAC
)
◆
Multiple mode separation is achieved on Sunrise series
Sunrise C18 and C18-SAC
Silanol Activity Controlled C18 HPLC Column
Sunrise C18-SAC is bonded with octadecylsilane on
a pure silica gel and controlled its silanol activity
without end-capping. Its carbon content is 14%.
Separation on Sunrise C18-SAC is done including
hydrogen bond and ion-exchange interaction
based on silanol groups except for hydrophobic
interaction. Control of pH and salt concentration of
a mobile phase can regulate retention.
0 2 4 6 8 10 12 14 16 18 20 22 24 26
retention time / min
3
1 2
4
5
3
1
2
4
5
3
1
2
4
5
3
1
2
4
5
100mM
200mM
50mM
25mM
Column size: 4.6x150 mm
Mobile phase: 70/30=CH
3
CN
/acetate buffer (pH4.1)
Flow rate: 1.0 mL/min
Temperature: 40
°
C
Sample:
1 = uracil
2 = toluene
3 = propranolol
4 = nortriptyline
5 = amitriptyline
10mM
3
1 2
4
5
Inner
diameter
length
Sunrise C18, 5μm Sunrise C18, 3μm Sunrise C18-SAC, 5μm Sunrise C18-SAC, 3μm
Cat. No.
Cat. No.
SA2241
75
－
SB2251
－
SA2251
4.6
10
SB3411
SB2411
SA3411
SA2411
10.0
250
SB3781
－
SA3781
－
20.0
250
SB3881
－
SA3881
－
75
－
SB2451
－
SA2451
SA2261
SA2271
SA2441
SA2461
SA2471
－
SB2241
SB2261
SB2271
SB2441
SB2461
SB2471
－
[mm]
[mm]
Cat. No.
Cat. No.
100
SB3261
SA3261
100
SB3461
SA3461
150
SB3471
SA3471
250
SB3481
SA3481
2.0
50
SB3241
SA3241
150
SB3271
SA3271
50
SB3441
SA3441
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