Basic HTML Version
Surface Roughness on Inner Surface of Column
Column: SunShell C18 30 x 3.0 mm. Mobile phase: A) Wtaer, B)
Acetonitrile; Gradient (Acetonitorile %), 0.00 min - 35%, 0.40 min - 100%,
0.80 min - 100%, 0.85 min - 35%, 1cycle; 1.8min, (High-pressure
gradient). Flow rate: 1.0 mL/min. Temperature: 40 ºC. Injection Volume: 1
µL. Wavelength: 200 - 500nm, CH-9, 215 - 500nm (Max Abs.). Sample:
Mixture of ultraviolet absorbers,
1 = 2,2’,4,4’-Tetrahydroxybenzophenone,
2 = Ethyl
p
-aminobenzoate, 3 = 2, 4-Dihydroxybenzophenone,
4 = 2,2’-Dihydroxy-4-methoxybenzophenone,
5 = 2,2’-Dihydroxy-4,4’-dimethoxybenzophenone,
6 = 2-Hydroxy-4-methoxybenzophenone,
7 = 2-(2’-Hydroxy-5’-metylphenyl) benzotriazole,
8 = 4-tert-Butylphenyl salicylate.
Courtesy of Jasco.
High-throughput separation
8 kinds of compounds were separated using SunShell C18 30 x 3.0 mm column in one minute.
A peak width of just one second
!!
Parameter of surface roughness
Ra: Average roughness from center line
Rz: Roughness calculated from 10 points average (5 points
of maximum and 5 points of minimum )
G company
Y company
S1 company
S2 company W company ChromaNIk Technologies
Ra
0.34
µ
m 0.32
µ
m
0.37
µ
m
0.03
µ
m 0.20
µ
m
0.01
µ
m
Rz
1.88
µ
m 1.62
µ
m
1.91
µ
m
0.19
µ
m 0.90
µ
m
0.10
µ
m
Center line
Ra
R
ｚ
It is considered that surface roughness affects column performance. Surface asperity of ChromaNik
Technologies column with 2.1 mm i.d. and 3.0 mm i.d. is 1/30 to 1/20 to compare with that of GL Sciences,
YMC, Shimadzu and Waters columns. ChromaNIk Technologies provides a column with a very smooth surface
which is the most suitable for 2.6
µ
m core shell particle packings.
2.6
µ
m
particle
2.6
µ
m
particle
Inner surface of column of A, B, C companies
Inner surface of column of ChromaNik Technologies
Schematic diagram of surface roughness
Website : www.chromtech.net.au E-mail : info@chromtech.net.au TelNo : 03 9762 2034 . . . in AUSTRALIA