Basic HTML Version
SunShell
の基本特性
Core shell silica
Bonded phase
Particle size
(
µ
m)
Pore diameter
(nm)
Specific surface
area (m
2
/g)
Carbon content
(%)
Bonded phase
Maximum operating
pressure
Available pH
range
SunShell C18 2.6
9
150
7
C18
60 MPa or 8,570 psi
1.5 - 10
SunShell C8
2.6
9
150
4.5
C8
60 MPa or 8,570 psi
1.5 - 9
SunShell PFP
2.6
9
150
4.5
Pentafluorophenyl
60 MPa or 8,570 psi
2 - 8
0 2 4 6 8 10 12 14 16 18 20 22 24
Retention time/min
Column:
SunShell PFP, 2.6
µ
m 150 x 4.6 mm
SunShell C8, 2.6
µ
m 150 x 4.6 mm
SunShell C18, 2.6
µ
m 150 x 4.6 mm
Mobile phase: CH
3
OH/H
2
O=75/25
Flow rate: 1.0 mL/min
Temperature: 40
º
C
Sample: 1 = Uracil, 2 = Caffeine, 3 = Phenol, 4 = Butylbenzene
5 = o-Terphenyl, 6 = Amylbenzene, 7 = Triphenylene
Retention of standard samples was compared for three kinds of phases such as C18, C8 and PFP. C18 showed the highest
Hydrogen bonding
(Caffeine/Phenol)
Hydrophobicity
(Amylbenzene/Butylbenzene)
Steric selectivity
(Triphenylene/o-Terphenyl)
SunShell PFP
1.00
1.31
2.38
SunShell C8
0.32
1.46
1.08
SunShell C18
0.39
1.60
1.46
SunShell PFP
SunShell C8
SunShell C18
1
1
1
2
2
7
3
2,3
3
4
4
4
5
5
5
6
6
6
7
7
SunShell C8, PFP
(Pentafluorophenyl)
◆
Characteristics of SunShell
◆
Comparison of standard samples
hydrophobicity and PFP showed both the highest steric selectivity and the highest hydrogen bonding. The feature of PFP phase is
to have hydrogen bonding, dipole-dipole interaction, aromatic and pi-pi interactions and hydrophobicity, which causes a different
selectivity from a C18 phase.
Separation of amitriptyline using C8 Separation of xanthines
Separation of isomers of xylene
SunShell C8 showed a sharp peak for
amitriptyline a well as SunShell C18.
SunShell PFP can retain xanthines more than
SunShell C18. The higher the concentration of
buffer, the longer the retention time.
SunShell PFP showed the different selectivity
from SunShell C18.
0 1
2
3
4
5
Retention time/min
SunShell C18, PFP 2.6
µ
m 150 x 2.1 mm
Mobile phase: CH
3
OH/water or buffer=30/70
Flow rate: 0.3 mL/min
Temperature: 25
º
C
Detection: UV@250nm
Sample: 1 = Theobromine
2 = Theophyline
3 = Caffeine
4 = Phenol
SunShell C8, 2.6
µ
m 150 x 4.6 mm
Mobile phase:
CH
3
CN/20mMphosphate buffer H7.0=60/40
Flow rate: 1.0 mL/min
Temperature: 40
º
C
Detection: UV@250nm
Sample: 1 = Uracil
2 = Propranolol
3 = Nortriptyline
4 = Amitriptyline
1 2
3
4
0 1
2
3
4
5
Retention time/min
SunShell
C18
SunShell
PFP
SunShell
PFP
SunShell
PFP
Water
Water
20mM
phosphate
buffer pH7
50mM
phosphate
buffer pH7
1
2
3
4
1
2
3
4
1 2
3
4
1
2
3
4
SunShell C18, PFP 2.6
µ
m 150 x 2.1 mm
Mobile phase:
CH
3
OH/water=75:25 for SunShell C18
CH
3
OH/water=60:40 for SunShell PFP
Flow rate: 0.3 mL/min
Temperature: 25
º
C
Detection: UV@250nm
Sample: 1 = o-Xylene
2 = m-Xylene
3 = p-Xylene
3
0 1
2
3
4
5
Retention time/min
SunShell C18
SunShell PFP
1
2
1
2
3
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