Table IIIa. Conditions for theSPE extraction, ctd.
1. 1 x 2mL acidic elution solution*; allow to soak into bed for up to 1
minute; followwith2 x 2mLmore solution.
2. Pass solutions through bed at a slow, dropwise rate into
prepared collection vessels.
#
3. Neutralize sampleswith5-7uL of ammonium hydroxide (check
using pHpaper) and correct final volume to 5mL before analysis.
* 1mL 85%H
3
PO
4
diluted to 1liter with deionizedHPLC gradewater
(0.1% solution)
# collection and analytical vesselsmust be deactivated before use
Extraction
