MALDI
• Matrix-assisted laser desorption/ionization
–Sample is depositedona target and co-
crystallizedwitha solidmatrix
(dihydroxybenzoic acid)
–Desorption/ionizationoccurs using a laser
suchasNd-YAG (266nm, v
4
)
–Energy transferred tomatrix, thenanalyte
–Useful inexcess of 200kDa
(biomacromolecules)
