Restek 2011/12 Chromatography Catalog - page 70

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GC COLUMNS | APPLICATION-SPECIFIC COLUMNS
Foods, Flavors & Fragrances
FAMEs (marine oil standard) on a FAMEWAX column.
similar phase
Omegawax
Peak List Conc. (µg/mL)
1. C14:0 600
2. C14:1 100
3. C16:0 1,600
4. C16:1 500
5. C18:0 800
6. C18:1 (oleate) 1,300
7. C18:1 (vaccenate) 400
8. C18:2 200
9. C18:3 200
10. C20:0 100
11. C20:1 900
12. C20:2 100
13. C20:4 300
14. C20:3 100
15. C20:5 1,000
16. C22:0 100
17. C22:1 300
18. C24:0 100
19. C22:6 1,200
20. C24:1 100
Column: FAMEWAX, 30m, 0.32mm ID, 0.25µm (cat.# 12498)
Inj.: 1µL
Conc.: 10,000µg/mL in isooctane
(total FAMES; see breakdown in peak list)
Oven temp.: 195–240°C at 5°C/min., 1 min. hold
Inj./det. temp.: 250°C/275°C
Carrier gas: hydrogen
Flow rate: 3mL/min. (constant flow)
Split ratio: 100:1
GC_FF00499
Polyunsaturated FAME Analysis
Column
engineered for
optimal resolution.
FAMEWAX Columns (fused silica)
(polar phase; Crossbond® polyethylene glycol)
• Application-specific columns for FAMEs, specially tested with a FAME mixture.
• Temperature range: 20 °C to 250 °C.
The elution order of polyunsaturated FAMEs on FAMEWAX columns is comparable to
that on other Carbowax® columns, but baseline resolution is achieved in significantly
less time.
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ID df temp. limits 30-Meter
0.25mm 0.25µm 20 to 250°C 12497
0.32mm 0.25µm 20 to 250°C 12498
0.53mm 0.50µm 20 to 250°C 12499
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