57
GC COLUMNS |
GENERAL PURPOSE COLUMNS
Rt®-2330, Rt®-2560
similar
phases
DB-23, HP-23, SP-2330, SP-2380
similar
phases
SPB-2560, HP-88, Silar 10C, CP-Sil 88 FAME,
CP-Sil 88
NLEA FAMEs resolved on an Rt®-2560 column.
Column:
Rt
®
-2560, 100m, 0.25mm ID,
0.20µm (cat.# 13199)
Sample:
NLEA FAME Mix (cat.# 35078),
30mg/mL total FAMEs in
methylene chloride
Inj.:
1.0µL split (split ratio 100:1),
4mm inlet liner (cat.# 20814)
Inj. temp.:
225°C
Carrier gas:
hydrogen, constant flow
Flow rate:
1.2mL/min.
Oven temp.:
100°C (4 min. hold) to 240°C
@ 3°C/min. (10 min. hold)
Det.:
FID @ 250°C
1. C4:0 methyl butyrate
2. C6:0 methyl hexanoate
3. C8:0 methyl octanoate
4. C10:0 methyl decanoate
5. C11:0 methyl undecanoate
6. C12:0 methyl laurate
7. C13:0 methyl tridecanoate
8. C14:0 methyl myristate
9. C14:1 methyl myristoleate (
cis
-9)
10. C15:0 methyl pentadecanoate
11. C16:0 methyl palmitate
12. C16:1 methyl palmitoleate (
cis
-9)
13. C17:0 methyl heptadecanoate
14. C18:0 methyl stearate
15. C18:1 methyl elaidate (
trans
-9)
16. C18:1 methyl oleate (
cis
-9)
17. C18:2 methyl linoelaidate (
trans
-9,12)
18. C18:2 methyl linoleate (
cis
-9,12)
19. C20:0 methyl arachidate
20. C20:1 methyl eicosenoate (
cis
-11)
21. C18:3 methyl linolenate (
cis
-9,12,15)
22. C22:0 methyl behenate
23. C22:1 methyl erucate (
cis
-13)
24. C23:0 methyl tricosanoate
25. C24:0 methyl lignocerate
26. C20:5 methyl eicosapentaenoate
(
cis
-5,8,11,14,17)
27. C24:1 methyl nervonate (
cis
-15)
28. C22:6 methyl docosahexaenoate
(
cis
-4,7,10,13,16,19)
GC_FF00651
(CH
2
)
3
Si
C N
90%
O
C N
(CH
2
)
3
Si
C N
10%
O
Rt®-2330 Structure
Doing Dioxin Analysis?
Rtx®-Dioxin2 columns provide better resolution
and higher maximum temperatures than
conventional columns. See
page 96
.
Resolves
cis
and
trans
FAME isomers
*Maximum temperatures listed are for 15- and 30-meter lengths. Longer lengths may have a slightly reduced
maximum temperature.
Rt®-2330 Columns
(fused silica)
(highly polar phase; 90% biscyanopropyl/10% phenylcyanopropyl polysiloxane—not bonded)
• General purpose columns for
cis
/
trans
FAMEs, dioxin isomers.
• Temperature range: 0 °C to 275 °C.
• Equivalent to USP G8 and G48 phase.
Rt®-2330 is one of the most polar capillary column stationary phases. Cyano groups on
both sides of the polymer backbone give the phase a strong dipole moment and high
selectivity for
cis
/
trans
compounds or compounds with conjugated double bonds.
Highly polar columns typically exhibit poor column efficiencies, high bleed, and short
column lifetimes when thermally cycled. To overcome some of these problems, we
developed a surface treatment that is more compatible with the Rt®-2330 phase. In
addition, our improved polymer produces columns with improved column efficiency
and lower bleed.
Because the Rt®-2330 stationary phase is not bonded, it should not be solvent rinsed.
Rt®-2560 Column
(fused silica)
(highly polar phase; biscyanopropyl polysiloxane—not bonded)
• Application-specific column for
cis
/
trans
FAMEs.
• Stable to 250 °C.
Because the Rt®-2560 stationary phase is not bonded, it should not be solvent rinsed.
ID
df
temp. limits*
30-Meter
60-Meter
105-Meter
0.25mm 0.10µm 0 to 260/275°C
10708
10711
10714
0.20µm 0 to 260/275°C
10723
10726
10729
0.32mm 0.20µm 0 to 260/275°C
10724
10727
10730
0.53mm 0.10µm 0 to 260/275°C
10710
10713
0.20µm 0 to 260/275°C
10725
10728
ID
df
temp. limits
10-Meter
20-Meter
40-Meter
0.18mm 0.10µm 0 to 260/275°C 40701
40702
40703
ID
df
temp. limits
100-Meter
0.25mm 0.20µm 20 to 250°C 13199
Website :
E-mail :
TelNo : 03 9762 2034 . . . in AUSTRALIA
Mar 2011
