MicroTee&Crossfor
CapillaryTubing
XX
Direct connect 1/16”, 1/32”, 360µmOD tubing, plus other
capillary tubing
XX
Low swept volume
UseUpchurchScientific
®
MicroTeesandMicroCrosses to joincapillary
tubing. All of these products aremade entirely of PEEKand have
0.006” (0.150mm) thru-holes, with resulting swept volumes ranging
from29 to81nL.
APPLICATIONNOTE
Several researchersuseour PEEKMicroTee to introduce ionizing voltage
to their fluid stream justprior toaMassSpectrometer
1
.MicroTeesare
well suited for thisapplicationdue toadvantageous internal geometry
andPEEKpolymer’selectrical resistance. Thematerials required for this
setupareas follows: onegoldorplatinumconductingwire, oneP-775
or P-875MicroTee (thispage), oneMicroTight TubingSleeve (page19)
for theconductingwire (asneeded toaccommodatewirediameter), and
at least twomoreMicroTight TubingSleeves (page19) toconnect your
capillary tubing.
To set upa similar connection, first thread yourwire through the
appropriate tubing sleeve, if necessary,with thewireextendingbeyond
bothendsof the sleeve. Slip the femalenut includedwith theMicroTee
over thewireor sleevedwire, followedby the ferrule—ensuring the
wire (and its sleeve) extendswell past theendof the ferrule tip.Align the
tipof thewirewith the thru-holeof theMicroTeeandgently insert the
wireuntil itbottomsout.Nowfinger tighten the femalenut intoplace.
Attach your flowpath tubing to theMicroTee’s twoother availableports,
following the instructionsprovidedwith theMicroTee.
Beginfluidflow through the teeandapply voltage to theconducting
wire lead. This setup typicallyprovideseffectiveelectrospray ionization in
applicationshavingaflow rateof 100µL/minorgreater.
1
One suchpaperdescribingpioneeringelectrospraywork:
Protein Identificationat theLowFemtomole
Level fromSilver-StainedGelsUsingaNewFritlessElectrospray Interface forLiquidChromatography-
Microspray and NanosprayMass Spectrometry.
Christine L. Gatlin, Gerd R. Kleemann, LaraG. Hays,
AndrewJ. Link, JohnR. Yates III (1998)Analytical Biochemistry263, 93-101.
PartNo. Description
Threads
Includes
Thru-hole
SweptVolume PressureRating
MICROTEE,MICROCROSSANDMICROELBOW
P-775
PEEKMicroTee forMicroTightSleeves
5/16-24Coned
(3) F-172, (3) P-416
0.006” (0.150mm)
29nL
4,000psi (276bar)
P-777
PEEKMicroCross forMicroTightSleeves
5/16-24Coned
(4) F-172, (4) P-416
0.006” (0.150mm)
38nL
4,000psi (276bar)
P-875
PEEKMicroTeewithMountingHole, forMicroTightSleeves
5/16-24Coned
(3) F-172, (3) P-416
0.006” (0.150mm)
29nL
4,000psi (276bar)
P-885
PEEKMicroTee for 1/32”ODTubing
5/16-24Coned
(3) F-112, (3) P-416
0.006” (0.150mm)
29nL
5,000psi (345bar)
P-887
PEEKMicroCross for 1/32”ODTubing
5/16-24Coned
(4) F-112, (4) P-416
0.006” (0.150mm)
38nL
5,000psi (345bar)
P-888
PEEKMicroTee for 360µmODTubing
5/16-24Coned
(3) F-152, (3) P-416BLK 0.006” (0.150mm)
29nL
5,000psi (345bar)
P-889
PEEKMicroCross for 360µmODTubing
5/16-24Coned
(4) F-152, (4) P-416BLK 0.006” (0.150mm)
38nL
5,000psi (345bar)
P-890
PEEKMicroTee for 1/16”ODTubing
5/16-24Coned
(3) F-132, (3) P-416
0.006” (0.150mm)
58nL
5,000psi (345bar)
P-891
PEEKMicroCross for 1/16”ODTubing
5/16-24Coned
(4) F-132, (4) P-416
0.006” (0.150mm)
81nL
5,000psi (345bar)
P-888
MicroTee
for 360µmOD tubing
0.006” thru-holes
withfittings included
P-890
MicroTee
for 1/16”OD tubing
0.006” thru-holes
withfittings included
P-775
MicroTee forMicroTight
®
TubingSleeves
0.006” thru-holes
withfittings included
(tubing sleevesnot included)
P-887
MicroCross
for 1/32”OD tubing
0.006” thru-holes
withfittings included
NOTE
XX
Useonly the ferrules suppliedwitheach connector— they are
not interchangeable. Replacement ferrules and femalenuts
are availableonpage 18. ForMicroUnions,MicroTees, and
MicroCrosses for UHPLC applications, seepage 42.
Biocompatible
1.19”
(3.02cm)
0.80”
(2.03cm)
1.19”
(3.02cm)
0.80”
(2.03cm)
1.19”
(3.02cm)
0.80”
(2.03cm)
1.19”
(3.02cm)
1.19”
(3.02cm)
CONNECTORS
45
HighPressureCapillary Tees&Crosses
