restek-tnx14 - page 98

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15
LemonOil
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The Rt-βDEXsm is the optimum col-
umn for obtaining chiral profiles of
Lemon and other citrus oils since it
provides enantiomeric separation for
themain terpene constituents like α-
and β-pinenes, sabinene (theseenan-
tiomers overlap with those of β-
pinene) and limonene (Figure 16).
RosemaryOil
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Chiral constituents in thisoil include
α-pinene, β-pinene, camphene, li-
monene, linalool, camphor, terpinen-
4-ol, α-terpineol, borneol, and
isoborneol. Baseline enantiomeric
separation is easilyachieved for all of
these compounds on the new Rt-
β DEXsm column. The common
permethylated β-cyclodextrincolumn
cannot completely resolve the optical
isomers of limonene, linalool, and
camphor ( Figure 17).
Figure 17
The Rt- βDEXsm, the most versatile β-cyclodextrin column for essential oil
analysis, resolves enantiomers of α-pinene, β-pinene, camphene, limonene,
linalool, camphor, terpinen-4-ol, α-terpineol, borneol, and isoborneol in
rosemary oil.
10 min. 0 20
1
30 40
2
3
3
4
4 1. (-/+)α-pinene
2. (+/-)camphene
3. (+/-)β-pinene
4. (-/+)limonene
5. eucalyptol (cineole)
6. linalool
7. camphor
8. (-/+)terpinen-4-ol
9. isoborneol
10. borneol
11. α-terpineol
6
6
7
7
8
8
9
9 10
10
11
11
5
30m, 0.32mm ID, 0.25µmRt- βDEXsm (cat.# 13104);
Oven temp.: 40°C (hold 1min.) to 200°C@ 2°C/min. (hold 3min.);
Carrier gas: hydrogen; 80cm/sec.; Detector: FID set @ 220°C
Figure 16
The Rt- βDEXsm column provides chiral resolution of the primary
terpenes in Artificial Lemon oil.
15 min. 10 20 25
1
30 35 40 45
1
2
2
3
3
4
4
1. (-/+)α-pinene
2. sabinene
3. (+/-)β-pinene
4. (-/+)limonene
30m, 0.32mm ID, 0.25µmRt- βDEXsm (cat.# 13104);
Oven temp.: 40°C (hold 1min.) to 200°C@ 2°C/min. (hold 3min.);
Carrier gas: hydrogen; 80cm/sec.; Detector: FID set @ 220°C
The Rt- βDEXsm is the
optimum column for
obtaining chiral profiles
of Lemonand other
citrus oils.
Visit Restek on-line at
www.restekcorp.com,
or call 800-356-1688,
ext. 4, for technical
assistance.