Nutraceuticals
Analyzing Nutraceutical Products by Liquid and Gas Chromatography
(lit. cat.# 59364)
HPLC andGCmethodologiesmeet the varied challenges of herbal products analysis
HPLC analyses of allicin in garlic, hyperforin in St. John'swort, and phenolics in Echinacea, andGC
analysis of fatty acids in saw palmetto, described in our 4-page note, showHPLC andGC to be excel-
lent tools for analyzingmarker compounds in botanical materials.
The Institute for Nutraceutical Advancement (INA) Validates GCMethods for SawPalmetto, Using
Rtx
®
-5 and Stabilwax
®
Columns
(lit. cat.# 59136)
Gas chromatography is a useful tool formonitoringmarker compounds in saw palmetto
Among themethods established by the INA are capillary GC analyses of fatty acids (as FAMEs) and of
phytosterols in saw palmetto. This 2-page note presents the analytical conditions for these analyses,
using Stabilwax
®
andRtx
®
-5 columns, respectively.
Determination of Omega-3 (n-3) and Omega-6 (n-6) Fatty Acid Composition in Evening Primrose
Oil, Flax Seed Oil, Black Currant Oil, and Borage Oil
(lit. cat.# 59128)
Capillary GC is an ideal approach tomonitoring essential fatty acids in plant oils
This 4-page note describes and illustrates capillary GC analyses of omega-3 and o
mega-6 essential fatty acids in plant oils. The polar polyethylene glycol-based column resolves satu-
rated andmonounsaturated FAMEs from the EFAs of interest, and resolves the isomers of linolenic
acid aswell.
HPLCAnalysis of Glucosinolates in Vegetable Extracts, without Ion Pairing, Using an Ultra
Aqueous C18 Column
(lit. cat.# 59335)
Convenient, reproducible analyses for hydrophilicmoleculeswithwidely varying polarity
Glucosinolateswith hydrophilic functional groups are very difficult to retain by conventional reversed
phase HPLC, andwhen the functional groups in a sample are diverse, polarity can differ widely, com-
plicating the problem. Until now, reversed phase HPLCwith an ion pairing reagent has been the
inconvenient, but necessary, approach to these analyses. This 2-page note describes a column and
conditions for analyses of glucosinolates, without ion pairing.
Foods
Analysis of Cholesterol and Other Dietary Sterols
(lit. cat.# 59581)
Analyzemany sterolswithout derivatization, using inert capillary GC columns
Capillary GC is a time-saving, efficient, and accurate approach for qualitative
and quantitative analyses of sterols. Nonpolar stationary phases generally are
suitable formonitoring dietary sterols, but amore polar column sometimes is
needed to resolve complexmixtures. This 2-page note describes and illus-
trates analyses of derivatized and underivatized sterols.
Analyzing Free Fatty Acids
(lit. cat.# 59583)
Avoid derivatizing samples and save time, effort, and expense
Typical sample preparation procedures for fatty acids analysis call for deriva-
tizing these analytes, but a properly designedGC analysis of the free acids
saves valuable time, effort, andmaterials. Read this 4-page note for informa-
tion about selecting a column, and steps to take to ensure accurate results,
when analyzing free fatty acids.
High-Resolution Analyses of Fatty AcidMethyl Esters (FAMEs) by Gas
Chromatography
(lit. cat.# 59489A)
Characterizing fats and oils, or determining fat content in food, calls for highly efficient
separations of fatty acidmethyl esters (FAMEs) on capillary GC columns. A properly chosen
column can provide accurate information about total fat,
trans
fat, or total omega-3 polyunsaturate content.
Polyethylene glycol-type stationary phases typically are used for separating, identifying, and quantifying saturated and unsaturated FAMEs. More polar biscyanopropyl
phases are needed to resolve
cis
and
trans
isomers of polyunsaturates or quantify total
trans
fat. This 4-page note discusses these challenging separations.
HPLCAnalysis of Vitamins
(lit. cat.# 59181)
Monitor fat-soluble or water-soluble vitamins in foods or dietary supplements
The need to accurately quantify vitamins in food products and dietary supplements necessitates simple, reliable, and accurate analytical procedures. Our 2-page note
describes anHPLC column and conditions for analyzing fat-soluble vitamins and equivalent information for analyzingwater-soluble vitamins.
Detection of Synthetic andNatural Antioxidants in Food
(lit. cat.# 59582)
Analyze antioxidants, using capillary GC
Phenolic antioxidants (BHA, BHT, etc.) can be analyzed simultaneously—quickly and effectively—on an intermediate-polarity capillary GC column. In addition, inert capil-
lary GC columns save time in tocopherols analysis because the analytes do not have to be derivatized. This 2-page note describes analytical columns and conditions for
antioxidants analyses.
Analysis of Preservatives, Using HPLC
(lit. cat.# 59398)
Optimize retention and selectivity
HPLC is a powerful tool for quantifying these compounds in food and beverage products. Analyses illustrated in this 6-page note include benzoic and sorbic acids,
parabens, phenolic antioxidants, and tocopherols.
