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Capillary GC stationary phases for organochlorine pesticides (e.g. US EPA
Method8081)must possess the selectivityneeded to resolve target pesticides,
yet withstand the rigors of repeated injections of extracts containing harsh
residuals from the samplematrix.TheUSEPAmethodalso requires a suitable
counterpart column for confirmation, to quantify potential pesticide “hits.”
An efficientway tomeet these requirements is throughdual column analysis,
which eliminates the need for a separate confirmation run, orGC/MS analy-
sis. A newly enhanced version of our Rtx®-CLPesticides2 column, with
dimensions of 30m x 0.32mm ID x 0.50µm (cat.#11325) is now available for
this application. The Rtx®-CLPesticides2 column, coupledwith anRtx®-440
column of the same dimensions (cat.# 12939), can provide a complete sepa-
rationof the 20most commonly analyzedorganochlorinepesticides, listed in
Method8081, in less than12minutes (Figure 1).
The unique selectivities of the Rtx®-CLPesticides2 column and the Rtx®-440
column enable the analysis to be run quickly, with good resolution andpeak
shapes, as shown inFigure1.Both columns produce similar run times,with a
set of elution order inversions and, because they share a common stationary
phase thickness, they should exhibit similar life expectancies. For accurate,
time-saving analyses of organochlorine pesticides, we highly recommend the
Rtx®-CLPesticides2 / Rtx®-440 column combination.
Rapid,DualColumnAnalysis forOrganochlorinePesticides
12-MinuteAnalysisUsingRtx®-CLPesticides2 / Rtx®-440CapillaryGCColumns
By JasonThomas, Environmental Innovations Chemist
• Analysis and confirmationwitha single injection.
• Rapidanalysis increases throughput.
• New, thicker Rtx®-CLPesticides2phase increases column lifetime.
Rtx®-CLPesticides2Column (fusedsilica)
ID
df (µm) temp. limits
length cat.#
0.32mm 0.50 -60 to 320/340°C 30-Meter 11325
Rtx®-440Column (fusedsilica)
ID
df (µm) temp. limits
length cat.#
0.25mm 0.25 20°C to 320/340°C 30-Meter 12923
SeCure™“Y”ConnectorKit
SeCure
™
“Y”connector body, 3knurlednuts,
“Y”Universal Press-Tight
®
union, 3 ferrules.
Ferrules Fit Column ID qty.
cat.#
0.28/0.32mm
kit
20277
OrganochlorinePesticideMixAB#2
20 components, listed in Figure 1, 8-80µg/mL in hexane:toluene
(1:1), 1mL/ampul
cat.# 32292
decachlorobiphenyl
2,4,5,6-tetrachloro-
m
-xylene
PesticideSurrogateMix
200µg/mL each in acetone, 1mL/ampul
cat.# 32000
Figure1
Sub-12-minutedual columnanalysisof organochlorinepesticides,
usinganRtx®CLPesticides2/Rtx®-440 columnpair.
GC_EV00777
1. 2,4,5,6-tetrachloro-
m
-xylene (surr.)
2.
α
-BHC
3.
γ
-BHC
4.
β
-BHC
5.
δ
-BHC
6. heptachlor
7. aldrin
8. heptachlor epoxide
9.
γ
-chlordane
10.
α
-chlordane
11. endosulfan I
12. 4,4'-DDE
13. dieldrin
14. endrin
15. 4,4'-DDD
16. endosulfan II
17. endrin aldehyde
18. 4,4'-DDT
19. endosulfan sulfate
20. methoxychlor
21. endrin ketone
22. decachlorobiphenyl (surr.)
GC_EV00776
Column:
Rtx
®
-CLPesticides2 30m, 0.32mm ID, 0.50µm (cat.# 11325) and Rtx
®
-440 30m, 0.32mm ID,
0.50µm (cat.# 12939) with 5m x 0.32mm ID Siltek
®
treated guard
tubing (cat.# 10027), connected using SeCure
™
“Y” Connector Kit (cat.# 20277) with
Universal “Y” Press-Tight
®
Connector
Sample:
OrganochlorinePesticideMix AB#2, 8-80µg/mL each component in hexane/toluene (cat.# 32292),
Pesticide SurrogateMix, 200µg/mL each component in acetone (cat.# 32000)
Inj.:
0.5µL splitless (hold 0.75min.), 3.5mm Siltek
®
treated single gooseneck inlet liner (cat.# 20961)
Inj. temp.:
250°C
Carrier gas:
hydrogen, constant pressure
Linear velocity: 60cm/sec.@ 300°C
Oven temp.:
110°C (hold 0.5min) to 230°C@ 25°C/min., to 330°C@ 15°C/min. (hold 1min.)
Det.:
ECD@ 330°C
Instrument:
ShimadzuGC-2010 FID/ECDwith AOC-20i autosampler
for
more
info
Formany additional pesticides
referencemixes, please refer to
our catalog, or visit ourwebsite.
