•
2
•
2005vol. 2
bioaccumulation (e.g., PCB#169).The list encom-
passes the seven European indicator compounds
and the 12most toxic congeners, according to the
WorldHealthOrganization (WHO) list.²
Usinga40mx0.18mm ID,0.18µm filmRtx®-PCB
column and the conditions listed in Figure 2, we
evaluated a sample composed of Aroclor® PCB
mixes 1242, 1254, and 1262. The 158 target PCB
congeners were identified in the sample, eluted as
135chromatographicpeaks.Of the158congeners,
110 eluted singly and48wereunresolved.
Produced through one of our newest polymer
technologies, Rtx®-PCB columns undergo rigor-
ous quality assurance measures, to ensure every
columnmeets exacting standards and to give you
highly reproducibleperformance, from column to
column. If you are analyzingPCBs—as congeners,
as Aroclor® mixtures, or as other mixtures (e.g.,
Kaneclor, Clophen, or Phenoclor mixes)—we
highly recommendusing thesenew columns.
Table I
110of 158 target PCB congeners inAroclor®mixes areeluted
singly fromanRtx®-PCB column.
PCB# s/m*
1
s
2
s
3
s
4
m
5
s
6
s
7
s
8
s
9
s
10
m
11
m
12
m
13
m
15
s
16
s
17
m
18
s
19
s
20
m
21
m
22
s
23
m
24
m
25
s
26
s
27
m
28
s
29
s
31
m
32
s
33
m
34
s
PCB# s/m*
35
s
37
s
40
s
41
s
42
s
43
s
44
s
45
s
46
s
47
s
48
s
49
s
51
m
52
s
53
m
54
m
55
s
56
m
57
m
59
s
60
m
63
s
64
s
66
s
67
s
70
s
71
s
74
s
75
s
76
m
77
m
81
s
PCB# s/m*
82
s
83
s
84
m
85
s
86
m
87
m
88
m
89
m
90
m
91
s
92
s
93
m
94
m
95
m
96
s
97
s
99
m
101
m
102
s
103
s
105
m
109
m
110
s
114
s
115
s
117
m
118
m
119
s
122
s
123
m
124
s
125
m
PCB# s/m*
126
s
128
m
129
s
130
s
131
m
132
s
133
s
134
s
135
s
136
m
137
s
138
s
139
s
141
s
144
s
146
s
147
m
149
m
151
s
153
s
154
m
156
s
157
s
158
s
163
s
164
s
166
s
167
s
169
s
170
s
171
s
172
s
PCB# s/m*
173
s
174
m
175
s
176
m
177
s
178
s
179
s
180
s
183
s
185
s
187
s
189
s
190
m
191
s
193
s
194
s
195
s
196
m
197
s
198
s
199
s
200
s
201
s
202
s
203
s
205
s
206
s
207
s
208
s
209
s
For additional PCB congenermixes, and Aroclor
®
reference
materials, please see our current catalog, or visit our website.
References
1. Frame, G., J. Cochran, and S., Bowadt,
Complete PCB Congener
Distributions for 17 AroclorMixtures Determined by 3HRGC Systems
Optimized for Comprehensive, Quantitative, Congener-Specific
Analysis
J. High Res. Chromatogr. 19, Dec. 1996, pp. 657-668.
2. Executive Summary,
Assessment of theHealth Risk of Dioxins:
Re-evaluation of the TolerableDaily Intake (TDI)
,WHO Consultation,
May 25-29, 1998, Geneva Switzerland. See:
Figure2
Excellent separationandpeak shape for PCBs in three
Aroclor®mixes, usinganRtx®-PCB column.
2,4,4' (BZ#28)
2,2',5,5' (BZ#52)
2,2',4,5,5' (BZ#101)
2,3',4,4',5 (BZ#118)
2,2',3,4,4',5' (BZ#138)
2,2',4,4',5,5' (BZ#153)
2,2',3,4,4',5,5' (BZ#180)
PCBCongenerStandard#2
10µg/mL each in isooctane, 1mL/ampul
cat.# 32294 (ea.)
$30
Rtx
®
-PCB (fusedsilica)
Themaximum temperatures listed are for 15- and 30-meter
lengths. Longer lengthsmay have a slightly reducedmaximum
temperature.
ID df (µm)
temp. limits
length cat.#
price
0.18mm 0.18 30°C to 320/340°C 20-Meter 41302
$360
0.18mm 0.18 30°C to 320/340°C 60-Meter 41304
$470
0.25mm 0.25 30°C to 320/340°C 30-Meter 13223
$445
0.25mm 0.25 30°C to 320/340°C 60-Meter 13226
$470
0.32mm 0.50 30°C to 320/340°C 30-Meter 13239
$470
*s - compound eluted singly; m - compound elutedwith one ormore other congeners.
Column:
Rtx
®
-PCB 40m, 0.18mm ID, 0.18µm (cat.# 41303)
Sample:
300ng/mL Aroclor
®
1242/1254/1262 in hexane: Aroclor
®
1242 (cat.# 32009),
Aroclor
®
1254 (cat.# 32011), Aroclor
®
1262 (cat.# 32409)
Inj.:
1.0µL splitless (hold 0.75min.), 4mm single gooseneck inlet liner (cat.# 20983)
Inj. temp.:
230°C
Carrier gas:
hydrogen, constant pressure
Linear velocity: 40cm/sec.@ 100°C
Oven temp.:
100°C (hold 1min.) to 200°C@ 30°C/min., to 320°C@ 2°C/min. (hold 1min.)
Det.:
ECD@ 330°C
GC_EV00768
min.
min.
