Figure4
Outstandingpeak symmetryeven for
difficult basic compounds
at 5.0ngonanRxi™-1ms column.
Unsurpassed Inertness
TheRxi™-1ms exhibits excellent inertness for both acidic andbasic compounds.We used 2, 4-dinitrophenol (acidic) andpyridine
(basic) to evaluate theRxi™-1ms column. This test is stringent,with anon-column amount of 0.5ng for each compound. Surface
activity in the column is revealedby the peak shapes and response factors for acidic andbasic compounds. The outstanding inert-
ness of theRxi™-1ms column allows acidic andbasic compounds tobe rununder the same conditions, as shownbelow.
6.576.586.596.606.616.626.636.646.656.666.676.686.696.706.716.726.736.74
2,4-dinitrophenol
m/z=184
RF=0.113
Figure2
Basic analytesonanRxi
™
-1ms column (0.5ng
each; extracted ion chromatograms).
Figure3
Acidic analyteonanRxi
™
-1ms column
(0.5ng; extracted ion chromatogram).
1,4-dioxane
(neutral)
m/z=88
RF=0.455
n-nitrosodimethylamine
(basic)
m/z=74
RF=0.651
pyridine
(basic)
m/z=79
RF=0.826
GC_EV00830
6.60 6.65 6.70
GC_EV00835
Column:
Rxi
™
-1ms, 30m, 0.25mm ID, 0.25µm (cat.# 13323)
Sample:
CustomAppendix IXMix, plus SV Internal StandardMix
(cat.# 31206), B/NSurrogateMix (4/89 SOW) (cat.# 31024),
Acid SurrogateMix (4/89 SOW) (cat.# 31025)
Inj.:
1.0µL, 5µg/mL each analyte (internal standards
25µg/mL), split (10:1)
4mmDrilledUniliner
®
inlet liner (holeat bottom) (cat.#20771)
Instrument:
Agilent 6890
Inj. temp.:
250°C
Carrier gas:
helium, constant flow
Flow rate:
1.2mL/min.
Oven temp.:
50°C (hold 0.5min.) to 300°C@ 25°C/min. (hold 5min.)
Det.:
Agilent 5973MSD
Transfer line
temp.:
280°C
Scan range:
35-550 amu
Solvent delay:
2.35min.
Tune:
DFTPP
Ionization:
EI
1. 2-fluorophenol (SS)
2. phenol-d6 (SS)
3. 1,4-dichlorobenzene-d4 (IS)
4. nitrobenzene-d5 (SS)
5. naphthalene-d8 (IS)
6. 2-fluorobiphenyl (SS)
7. acenaphthene-d10 (IS)
8. 1-naphthalenamine
9. 2-naphthalenamine
10. 5-nitro-
o
-toluidine
11. diphenylamine
12. 2,4,6-tribromophenol (SS)
13. 4-aminobiphenyl
14. phenanthrene-d10 (IS)
15.
p
-terphenyl-d14 (SS)
16.
p
-dimethylaminoazobenzene
17. 2-acetylaminofluorene
18. chrysene-d12 (IS)
19. perylene-d12 (IS)
Conclusion
The Rxi™-1ms column is the new industry standard for 100% dimethyl polysiloxane columns. Column bleed is the lowest in the
industry, improving compound signal tonoise ratios and allowing lower detection limits for active compounds. The unsurpassed
inertnessof thecolumnallowsbothacidicandbasiccompounds tobeanalyzed,oftenunder the sameconditions. Weguaranteeyou
will be 100% satisfiedwith the performance of theRxi
™
-1ms column.
Excellent peak shape
at lowanalyte levels.
Similar peak shape
demonstrates column
inertness.
