SNAP
®
SeriesGlass Columns, AB-Version, Short/Short Plungers
Operating theColumnwith the PackingAdaptor
Preparation:
The main column components in contact with the mobile phase must be cleaned thoroughly before the column is used for
the first time. Essential Life Solutions recommends dismantling the column and washing the components in an appropriate
laboratory cleaning solution prior to use. After cleaning, all components should be rinsed in double distilledwater and reas-
sembled.
Essential Life Solutions assumes that the column will be slurry packed. Slurry packing would involve mixing approximately
equal partsmobilephaseand stationary phaseoutsideof the columnprior topacking.
Consult with your supplier formedia’s
bulk density.
This will result in a volume approximately double the final packed volume.
V
= (π
R
2
H)
1000
V=volume (mL) R=radius of column (mm) H=desired height (mm)
m= V
x
BD
m=mass (grams) BD=bulk density (grams/mL)
Assemble the packing adaptor on top of the processing column. Clip split ring over glass column at 0mm end. Install the
compression nut so that the split ring is positioned partially inside. Insert seal ring with the small face down. Partially thread
theglass column/compressionnut assembly into thebody of thepackingadaptor.Open the jawandplaceonto the column’s
bare glass tube. Fasten the latch and fully tighten the compression nut.
See diagram below.
Column operation is initiated by attaching to an appropriate chromatographic system or pump using the supplied fittings.
Tubing size shouldbe selected toprovide appropriate flow conditions. Tubingmaterial shouldalsobe biocompatiblewith the
mobile phase selected. Connect the inlet tubing to the end of the piston and the controller unit. To prevent back pressure, do
not connect the outlet tubing to the controller unit and cap the end of the tubing.
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