page
In reversedphaseandadsorption chromatography, thepossibilities for usingglass columns areoften limiteddue to high
back pressures generatedby small particles. The high pressure resistance of the Econoline®glass columns allow you to
achieve high flow rates for demanding separations. The example shows that this enables a considerableaccelerationof
the separation, whichmeans that you can achieve significant time savings.
30ml/min
9 bar
N=2245
A
S
=1,32
75ml/min
28 bar
N=1490
A
S
=1,19
125ml/min
50 bar
N=1101
A
S
=1,22
Sample:
Uracil
0,175mg/ml
Methylbenzoate 1,25 µl/ml
Naphthalene 0,5mg/ml
Butyl benzoate 4,0 µl/ml
75% Time saving due to
high pressure resistance
= higher flows possible
Column:
ELS Econoline® 125 x 25mm ID
Stationary phase: YMC-PackODS-AQ, 10 µm, 12 nm, 8 cm bed length
Flow rate:
30ml/min - 75ml/min - 125ml/min
Mobile phase:
ACN/H
2
O (50:50)
Injection:
500 µl
Detection:
UV at 254 nm
Separation of a standard test mixture of proteins
Column:
ELS Econoline®250 x 15mm ID
Stationary phase: YMC-BioPro SP, 30 μm (bed length 170mm)
Mobile phase:
A) 20mMKH
2
PO
4
*K
2
HPO
4
(pH6.8)
B) 20mMKH
2
PO
4
*K
2
HPO
4
(pH6.8) containing0.5MNaCl
Gradient:
40-80% B
Flow rate:
6ml/min
Temperature:
25°C
Detection:
UVat 220 nm
Injection:
100 μl
Sample:
1. Ribonuclease A
2. CytochromeC
3. Lysozyme
Example of applicationwith reversed phasemedia
Application Examples
Econoline
®
Series Glass Columns
