9
Figure 17
Phenolic preservatives, includingBHA andBHT,
on a Pinnacle II
™
C18HPLC column.
Figure 16
Preservatives BHA andBHT on an Rtx
®
-50 intermediate polarity column.
Rtx
®
-50, 30m, 0.53mm ID, 0.50µm (cat.# 10540)
Sample:
50ppm each analyte in
methanol
Inj.:
1.0µL direct, gooseneck
splitless inlet liner, 4mm
(cat.# 20798)
Inj. temp.:
280°C
Carrier gas:
helium, constant pressure
Linear velocity: 60cm/sec.@ 50°C
Oven temp.:
50°C to 240°C@15°C/min.
(hold 3min.)
Det.:
FID@280°C
GC_FF00630
1. BHA
2. BHT
LC_0198
1
2
3
4
0
2
4
6
8
10
12
14
16
18min.
PeakList:
Conc. (ppm)
1. propyl gallate
168
2. TBHQ
182
3. 2-BHA+ 3-BHA 197
4. BHT
193
Sample:
Inj.:
10µL
Conc.:
see peak list
Solvent:
methanol
Column:
Pinnacle
II
™
C18
Catalog #:
9214565
Dimensions: 150 x 4.6mm
Particle size: 5µm
Pore size:
110Å
Conditions:
Mobile phase: A= 1% acetic acid
B=methanol
Time B
(min.) (%)
0 50
4 50
10 90
25 90
26 50
Flow:
1.0mL/min
Temp.:
30°C
Det.:
UV@280nm
Secure, Reliable Column-to
Column Connections
Use aVu2 Union
™
connector when
you:
• Connect a guard column to an analyti-
cal column.
• Connect a column to a transfer line or
restrictor line.
• Connect two columns in a series.
• Repair a broken column.
Vu2Union
™
Connector Kits
Kits include: Vu2Union
™
body, 2 knurled
nuts, 2Press-Tight
®
unions, and 4 ferrules
Fits Column ID
qty.
cat.#
0.15–0.25mm
kit
21105
0.28/0.32mm
kit
21106
0.45/0.50& 0.53mm
kit
21107
Knurled nut
2-pk.
21108
hanger
body
ferrule
knurled
nut
Press-Tight
®
Union
fused silica
column, guard
column or
transfer line
The Vu2Union
™
connector’s open design allows
visual confirmation of the seal; secondary seals
ensure a leak-tight connection.
Questions?
Contact Restek’s Technical Service
Team!We have answers to your
toughest analytical questions.
Call 800-356-1688 or 814-353-1300,
ext. 4, email us at
or contact your
local Restek representative.
Website :
E-mail
TelNo : 03 9762 2034 . . . inAUSTRALIA
